Your browser doesn't support javascript.
loading
Depletion of mitochondrial protease OMA1 alters proliferative properties and promotes metastatic growth of breast cancer cells.
Daverey, Amita; Levytskyy, Roman M; Stanke, Kimberly M; Viana, Martonio Ponte; Swenson, Samantha; Hayward, Stephen L; Narasimhan, Madhusudhanan; Khalimonchuk, Oleh; Kidambi, Srivatsan.
Afiliación
  • Daverey A; Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, NE, United States.
  • Levytskyy RM; Department of Biochemistry, University of Nebraska, Lincoln, NE, United States.
  • Stanke KM; Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, NE, United States.
  • Viana MP; Department of Biochemistry, University of Nebraska, Lincoln, NE, United States.
  • Swenson S; Department of Biochemistry, University of Nebraska, Lincoln, NE, United States.
  • Hayward SL; Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, NE, United States.
  • Narasimhan M; Department of Pharmacology and Neuroscience, Texas Tech University Health Sciences Center, Lubbock, TX, United States.
  • Khalimonchuk O; Department of Biochemistry, University of Nebraska, Lincoln, NE, United States. okhalimonchuk2@unl.edu.
  • Kidambi S; Nebraska Redox Biology Center, University of Nebraska, Lincoln, NE, United States. okhalimonchuk2@unl.edu.
Sci Rep ; 9(1): 14746, 2019 10 14.
Article en En | MEDLINE | ID: mdl-31611601
Metastatic competence of cancer cells is influenced by many factors including metabolic alterations and changes in mitochondrial biogenesis and protein homeostasis. While it is generally accepted that mitochondria play important roles in tumorigenesis, the respective molecular events that regulate aberrant cancer cell proliferation remain to be clarified. Therefore, understanding the mechanisms underlying the role of mitochondria in cancer progression has potential implications in the development of new therapeutic strategies. We show that low expression of mitochondrial quality control protease OMA1 correlates with poor overall survival in breast cancer patients. Silencing OMA1 in vitro in patient-derived metastatic breast cancer cells isolated from the metastatic pleural effusion and atypical ductal hyperplasia mammary tumor specimens (21MT-1 and 21PT) enhances the formation of filopodia, increases cell proliferation (Ki67 expression), and induces epithelial-mesenchymal transition (EMT). Mechanistically, loss of OMA1 results in alterations in the mitochondrial protein homeostasis, as reflected by enhanced expression of canonic mitochondrial unfolded protein response genes. These changes significantly increase migratory properties in metastatic breast cancer cells, indicating that OMA1 plays a critical role in suppressing metastatic competence of breast tumors. Interestingly, these results were not observed in OMA1-depleted non-tumorigenic MCF10A mammary epithelial cells. This newly identified reduced activity/levels of OMA1 provides insights into the mechanisms leading to breast cancer development, promoting malignant progression of cancer cells and unfavorable clinical outcomes, which may represent possible prognostic markers and therapeutic targets for breast cancer treatment.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Metaloendopeptidasas / Adenocarcinoma / Mitocondrias / Invasividad Neoplásica Tipo de estudio: Prognostic_studies Límite: Female / Humans Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Metaloendopeptidasas / Adenocarcinoma / Mitocondrias / Invasividad Neoplásica Tipo de estudio: Prognostic_studies Límite: Female / Humans Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido