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4D electron microscopy of T cell activation.
Lu, Yue; Yoo, Byung-Kuk; Ng, Alphonsus H C; Kim, Jungwoo; Yeom, Sinchul; Tang, Jau; Lin, Milo M; Zewail, Ahmed H; Heath, James R.
Afiliación
  • Lu Y; The Arthur Amos Noyes Laboratory of Chemical Physics, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125.
  • Yoo BK; Institute for Systems Biology, Seattle, WA 98109.
  • Ng AHC; The Arthur Amos Noyes Laboratory of Chemical Physics, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125; bkyoo@caltech.edu jheath@systemsbiology.org.
  • Kim J; The Arthur Amos Noyes Laboratory of Chemical Physics, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125.
  • Yeom S; Institute for Systems Biology, Seattle, WA 98109.
  • Tang J; The Arthur Amos Noyes Laboratory of Chemical Physics, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125.
  • Lin MM; The Arthur Amos Noyes Laboratory of Chemical Physics, Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125.
  • Zewail AH; Mechanical, Aerospace and Biomedical Engineering Department, University of Tennessee, Knoxville, TN 37996.
  • Heath JR; The Institute for Technological Sciences, Wuhan University, Wuhan 430072, China.
Proc Natl Acad Sci U S A ; 116(44): 22014-22019, 2019 10 29.
Article en En | MEDLINE | ID: mdl-31611413
T cells can be controllably stimulated through antigen-specific or nonspecific protocols. Accompanying functional hallmarks of T cell activation can include cytoskeletal reorganization, cell size increase, and cytokine secretion. Photon-induced near-field electron microscopy (PINEM) is used to image and quantify evanescent electric fields at the surface of T cells as a function of various stimulation conditions. While PINEM signal strength scales with multiple of the biophysical changes associated with T cell functional activation, it mostly strongly correlates with antigen-engagement of the T cell receptors, even under conditions that do not lead to functional T cell activation. PINEM image analysis suggests that a stimulation-induced reorganization of T cell surface structure, especially over length scales of a few hundred nanometers, is the dominant contributor to these PINEM signal changes. These experiments reveal that PINEM can provide a sensitive label-free probe of nanoscale cellular surface structures.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Activación de Linfocitos / Linfocitos T Límite: Humans Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Activación de Linfocitos / Linfocitos T Límite: Humans Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos