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CRISPR/Cas9 facilitates genomic editing for large-scale functional studies in pluripotent stem cell cultures.
Li, Xiao-Fei; Zhou, Yong-Wei; Cai, Peng-Fei; Fu, Wei-Cong; Wang, Jin-Hua; Chen, Jin-Yang; Yang, Qi-Ning.
Afiliación
  • Li XF; Department of Joint Surgery, Jinhua Municipal Central Hospital, No. 365 Renmin East Road, Wucheng District, Jinhua, 321000, Zhejiang, People's Republic of China.
  • Zhou YW; Department of Joint Surgery, Jinhua Municipal Central Hospital, No. 365 Renmin East Road, Wucheng District, Jinhua, 321000, Zhejiang, People's Republic of China.
  • Cai PF; Department of Joint Surgery, Jinhua Municipal Central Hospital, No. 365 Renmin East Road, Wucheng District, Jinhua, 321000, Zhejiang, People's Republic of China.
  • Fu WC; Department of Joint Surgery, Jinhua Municipal Central Hospital, No. 365 Renmin East Road, Wucheng District, Jinhua, 321000, Zhejiang, People's Republic of China.
  • Wang JH; Department of Joint Surgery, Jinhua Municipal Central Hospital, No. 365 Renmin East Road, Wucheng District, Jinhua, 321000, Zhejiang, People's Republic of China.
  • Chen JY; Research and Development Department, Zhejiang Healthfuture Institute for Cell-Based Applied Technology, Hangzhou, 310052, Zhejiang, People's Republic of China.
  • Yang QN; Department of Joint Surgery, Jinhua Municipal Central Hospital, No. 365 Renmin East Road, Wucheng District, Jinhua, 321000, Zhejiang, People's Republic of China. jhyangqn@sina.com.
Hum Genet ; 138(11-12): 1217-1225, 2019 Dec.
Article en En | MEDLINE | ID: mdl-31606751
Pluripotent stem cell (PSC) cultures form an integral part of biomedical and medical research due to their capacity to rapidly proliferate and differentiate into hundreds of highly specialized cell types. This makes them a highly useful tool in exploring human physiology and disease. Genomic editing of PSC cultures is an essential method of attaining answers to basic physiological functions, developing in vitro models of human disease, and exploring potential therapeutic strategies and the identification of drug targets. Achieving reliable and efficient genomic editing is an important aspect of using large-scale PSC cultures. The CRISPR/Cas9 genomic editing tool has facilitated highly efficient gene knockout, gene correction, or gene modifications through the design and use of single-guide RNAs which are delivered to the target DNA via Cas9. CRISPR/Cas9 modification of PSCs has furthered the understanding of basic physiology and has been utilized to develop in vitro disease models, to test therapeutic strategies, and to facilitate regenerative or tissue repair approaches. In this review, we discuss the benefits of the CRISPR/Cas9 system in large-scale PSC cultures.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Genómica / Células Madre Pluripotentes / Técnicas de Inactivación de Genes / Sistemas CRISPR-Cas / Edición Génica Límite: Humans Idioma: En Revista: Hum Genet Año: 2019 Tipo del documento: Article Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Genómica / Células Madre Pluripotentes / Técnicas de Inactivación de Genes / Sistemas CRISPR-Cas / Edición Génica Límite: Humans Idioma: En Revista: Hum Genet Año: 2019 Tipo del documento: Article Pais de publicación: Alemania