Construction of chromosome segment substitution lines of Dongxiang common wild rice (Oryza rufipogon Griff.) in the background of the japonica rice cultivar Nipponbare (Oryza sativa L.).

Ma, Xiaoding; Han, Bing; Tang, Jianghong; Zhang, Jiani; Cui, Di; Geng, Leiyue; Zhou, Huiying; Li, Maomao; Han, Longzhi

Ma, Xiaoding; Han, Bing; Tang, Jianghong; Zhang, Jiani; Cui, Di; Geng, Leiyue; Zhou, Huiying; Li, Maomao; Han, Longzhi.

Afiliación

Ma X; National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Han B; National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Tang J; Chongqing Normal University, Chongqing, 401331, China.
Zhang J; Chongqing Normal University, Chongqing, 401331, China.
Cui D; National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Geng L; National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Zhou H; Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang, 330200, Jiangxi, China.
Li M; Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang, 330200, Jiangxi, China. Electronic address: lmm3056@163.com.
Han L; National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. Electronic address: hanlongzhi@caas.cn.

Plant Physiol Biochem ; 144: 274-282, 2019 Nov.

Article en En | MEDLINE | ID: mdl-31593900

RESUMEN

Dongxiang common wild rice (Oryza rufipogon Griff.) is believed to have the northernmost distribution of any wild rice species. Many favorable genes were lost during rice domestication, and Dongxiang common wild rice is a potential source of many genes related to biological and abiotic stress resistance and high grain yield. Despite its importance, japonica rice has not yet been used as a background material for the construction of introgression lines carrying Dongxiang common wild rice chromosome segments. In this study, we used Dongxiang common wild rice as the donor parent and Nipponbare (Oryza sativa L), a reference-sequence japonica cultivar, as the recurrent parent to develop a set of 104 chromosome segment substitution lines (CSSLs) through crossing and backcrossing with marker-assisted selection based on 203 polymorphic molecular markers evenly distributed across 12 chromosomes. The 104 CSSLs covered 87.94% of the genome of Dongxiang common wild rice. Each CSSL contained an average of four introgressed segments, and the average segment length was 3.3â¯Mb. Quantitative trait locus (QTL) analysis of the panicle-related traits of the CSSLs at two sites (Beijing and Nanchang, Jiangxi Province) revealed 18 QTLs for eight traits. Among these 18 QTLs were two known grain length- and width-related genes and four novel QTLs. In addition, two QTLs were verified, and two novel QTLs were identified, for panicle neck length, a domestication-related trait. The developed CSSLs potentially represent a valuable population for detecting favorable genes in common wild rice and creating germplasm resources for japonica rice breeding.

Asunto(s)

Cromosomas de las Plantas/genética; Oryza/genética; Sitios de Carácter Cuantitativo/genética; Genoma de Planta/genética; Genotipo; Fenotipo

Palabras clave

Chromosome segment substitution lines; Japonica rice; Oryza rufipogon; Oryza sativa; Quantitative trait locus

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / Cromosomas de las Plantas / Sitios de Carácter Cuantitativo Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Physiol Biochem Asunto de la revista: BIOQUIMICA / BOTANICA Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Francia

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