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Impact of general anaesthesia on endoplasmic reticulum stress: propofol vs. isoflurane.
Seo, Eun-Hye; Piao, Liyun; Park, Hyun-Jun; Lee, Ji Yeon; Sa, Mijung; Oh, Chung-Sik; Lee, Seung-Hyun; Kim, Seong-Hyop.
Afiliación
  • Seo EH; BK21 Plus, Department of Cellular and Molecular Medicine, Konkuk University School of Medicine, Seoul, Korea.
  • Piao L; Department of Infection and Immunology, Konkuk University School of Medicine, Seoul, Korea.
  • Park HJ; Department of Infection and Immunology, Konkuk University School of Medicine, Seoul, Korea.
  • Lee JY; Department of Infection and Immunology, Konkuk University School of Medicine, Seoul, Korea.
  • Sa M; Department of Anesthesiology and Pain medicine, Konkuk University Medical Center, Konkuk University School of Medicine, Seoul, Korea.
  • Oh CS; Department of Anesthesiology and Pain medicine, Konkuk University Medical Center, Konkuk University School of Medicine, Seoul, Korea.
  • Lee SH; Department of Microbiology, Konkuk University School of Medicine, Seoul, Korea.
  • Kim SH; Department of Medicine, Institute of Biomedical Science and Technology, Konkuk University School of Medicine, Seoul, Korea.
Int J Med Sci ; 16(9): 1287-1294, 2019.
Article en En | MEDLINE | ID: mdl-31588195
Background: This study investigated the effects of propofol and isoflurane on endoplasmic reticulum (ER) stress in an animal model under general anaesthesia. Methods: Rats were randomly divided into Propofol and Isoflurane groups. Anaesthesia was maintained with propofol for Propofol group or isoflurane for Isoflurane group during 3 h. ER stress from lymphocytes in blood and tissues was evaluated between two groups after euthanasia. Reactive oxygen species (ROS) from lymphocytes in blood and tissues, and cytokines in blood were also checked. An immunohistochemical assay for ER stress marker from tissues was performed. Results: After anaesthesia, the levels of CCAAT-enhancer-binding protein homologous proteins (CHOP) in blood and liver were significantly higher in Isoflurane group, compared to Propofol group [blood, 31,499 ± 4,934 (30,733, 26,441-38,807) mean fluorescence intensity (MFI) in Isoflurane group vs. 20,595 ± 1,838 (20,780, 18,866-22,232) MFI in Propofol group, p = 0.002; liver, 28,342 ± 5,535 (29,421, 23,388-32,756) MFI in Isoflurane group vs. 20,004 ± 2,155 (19,244, 18,197-22,191) MFI in Propofol group, p = 0.020]. ROS in blood was significantly higher in Isoflurane group, compared to Propofol group. However, cytokines in blood and immunohistochemical assays in tissues were similar between groups. Conclusion: Significant higher of ER stress from blood and liver were observed in rats under anaesthesia with isoflurane, compared to those that received propofol. ROS from blood also showed significant higher under anaesthesia with isoflurane. However, these findings were not associated with any changes in cytokines in blood or immunohistochemical assay in tissues.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Propofol / Estrés del Retículo Endoplásmico / Isoflurano / Anestesia General Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int J Med Sci Asunto de la revista: MEDICINA Año: 2019 Tipo del documento: Article Pais de publicación: Australia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Propofol / Estrés del Retículo Endoplásmico / Isoflurano / Anestesia General Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int J Med Sci Asunto de la revista: MEDICINA Año: 2019 Tipo del documento: Article Pais de publicación: Australia