Structural insights into Acyl-coenzyme A binding domain containing 3 (ACBD3) protein hijacking by picornaviruses.

Chalupska, Dominika; Rózycki, Bartosz; Klima, Martin; Boura, Evzen

Chalupska, Dominika; Rózycki, Bartosz; Klima, Martin; Boura, Evzen.

Afiliación

Chalupska D; Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague, Czech Republic.
Rózycki B; Institute of Physics of the Polish Academy of Sciences, Warsaw, Poland.
Klima M; Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague, Czech Republic.
Boura E; Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague, Czech Republic.

Protein Sci ; 28(12): 2073-2079, 2019 12.

Article en En | MEDLINE | ID: mdl-31583778

RESUMEN

Many picornaviruses hijack the Golgi resident Acyl-coenzyme A binding domain containing 3 (ACBD3) protein in order to recruit the phosphatidylinositol 4-kinase B (PI4KB) to viral replication organelles (ROs). PI4KB, once recruited and activated by ACBD3 protein, produces the lipid phosphatidylinositol 4-phosphate (PI4P), which is a key step in the biogenesis of viral ROs. To do so, picornaviruses use their small nonstructural protein 3A that binds the Golgi dynamics domain of the ACBD3 protein. Here, we present the analysis of the highly flexible ACBD3 proteins and the viral 3A protein in solution using small-angle X-ray scattering and computer simulations. Our analysis revealed that both the ACBD3 protein and the 3A:ACBD3 protein complex have an extended and flexible conformation in solution.

Asunto(s)

Acilcoenzima A/química; Proteínas Adaptadoras Transductoras de Señales/química; Proteínas de la Membrana/química; Picornaviridae/química; Acilcoenzima A/metabolismo; Proteínas Adaptadoras Transductoras de Señales/metabolismo; Sitios de Unión; Humanos; Proteínas de la Membrana/metabolismo; Picornaviridae/metabolismo

Palabras clave

ACBD3; RNA virus; coarse-grained simulations; host factor; intrinsically disordered regions; picornavirus; small-angle X-ray scattering (SAXS)

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Picornaviridae / Acilcoenzima A / Proteínas Adaptadoras Transductoras de Señales / Proteínas de la Membrana Límite: Humans Idioma: En Revista: Protein Sci Asunto de la revista: BIOQUIMICA Año: 2019 Tipo del documento: Article País de afiliación: República Checa Pais de publicación: Estados Unidos

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