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Epidermophyton floccosum: nucleotide sequence analysis and antifungal susceptibility testing of 40 clinical isolates.
Ansari, Saham; Ahmadi, Bahram; Norouzi, Maryam; Ansari, Zohreh; Afsarian, Mohammad Hosein; Lotfali, Ensieh; Rezaei-Matehkolaei, Ali.
Afiliación
  • Ansari S; Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Ahmadi B; Department of Medical Laboratory Sciences, School of Para-Medicine, Bushehr University of Medical Sciences, Bushehr, Iran.
  • Norouzi M; Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Ansari Z; Department of Chemistry, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
  • Afsarian MH; Department of Medical Mycology and Parasitology, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran.
  • Lotfali E; Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Rezaei-Matehkolaei A; Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
J Med Microbiol ; 68(11): 1655-1663, 2019 Nov.
Article en En | MEDLINE | ID: mdl-31573466
Purpose. Epidermophyton floccosum is an anthropophilic dermatophyte species, which is one of the common causative agents of dermatophytosis in different parts of the world. The aim of the present investigation was to evaluate the genetic diversity of E. floccosum strains isolated from different parts of Iran and to define the in vitro susceptibility profiles of seven antifungal drugs against these clinical isolates.Methodology. Forty clinical strains of E. floccosum isolated from 40 patients with dermatophytosis were subjected to DNA extraction and PCR amplification of the ITS rDNA region using universal primers ITS1 and ITS4. The in vitro activities of griseofulvin, itraconazole, voriconazole, posaconazole, caspofungin, ketoconazole and terbinafine were determined using a broth microdilution method according to the CLSI-M-38A2 protocol.Results. A mean genetic similarity of 99.5 % was found between E. floccosum strains, with intraspecies differences ranging from 0 to 3 nt. The geometric mean (GM) MICs and minimum effective concentrations (MECs) across all isolates were, in increasing order, as follows: terbinafine (GM=0.018 mg l-1), posaconazole (GM=0.022 mg l-1), itraconazole (GM=0.034 mg l-1) and voriconazole (GM=0.045 mg l-1), which had low MICs against all tested strains, whereas caspofungin (GM=0.22 mg l-1), ketoconazole (GM=0.41 mg l-1) and griseofulvin (GM=0.62 mg l-1) demonstrated higher MICs.Conclusion. Our study showed low intraspecies variation within strains of E. floccosum. Furthermore, terbinafine, posaconazole, itraconazole and voriconazole were shown to be the most potent antifungal drugs against E. floccosum strains.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tiña / Epidermophyton / Antifúngicos Tipo de estudio: Guideline Límite: Humans País/Región como asunto: Asia Idioma: En Revista: J Med Microbiol Año: 2019 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tiña / Epidermophyton / Antifúngicos Tipo de estudio: Guideline Límite: Humans País/Región como asunto: Asia Idioma: En Revista: J Med Microbiol Año: 2019 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Reino Unido