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Soybean antiviral immunity conferred by dsRNase targets the viral replication complex.
Ishibashi, Kazuhiro; Saruta, Masayasu; Shimizu, Takehiko; Shu, Miao; Anai, Toyoaki; Komatsu, Kunihiko; Yamada, Naohiro; Katayose, Yuichi; Ishikawa, Masayuki; Ishimoto, Masao; Kaga, Akito.
Afiliación
  • Ishibashi K; Plant and Microbial Research Unit, Division of Plant and Microbial Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan.
  • Saruta M; Crop Breeding and Food Functional Components Division, Western Region Agricultural Research Center, National Agriculture and Food Research Organization, 1-3-1 Senyu-cho, Zentsuji-shi, Kagawa, 765-8508, Japan.
  • Shimizu T; Soybean Breeding Unit, Institute of Crop Science, National Agriculture and Food Research Organization, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8518, Japan.
  • Shu M; Soybean and Field Crop Applied Genomics Research Unit, Institute of Crop Science, National Agriculture and Food Research Organization, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8518, Japan.
  • Anai T; Advanced Genomics Breeding Section, Institute of Crop Science, National Agriculture and Food Research Organization, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8518, Japan.
  • Komatsu K; Plant and Microbial Research Unit, Division of Plant and Microbial Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan.
  • Yamada N; Faculty of Agriculture, Saga University, 1 Honjo-machi, Saga, 840-8502, Japan.
  • Katayose Y; Research Team for Crop Cold Tolerance, Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka 1, Toyohira, Sapporo, Hokkaido, 062-8555, Japan.
  • Ishikawa M; Crop Breeding and Food Functional Components Division, Western Region Agricultural Research Center, National Agriculture and Food Research Organization, 1-3-1 Senyu-cho, Zentsuji-shi, Kagawa, 765-8508, Japan.
  • Ishimoto M; Nagano Vegetable and Ornamental Crops Experiment Station, 1066-1, Soga, Shiojiri, Nagano, 399-6461, Japan.
  • Kaga A; Advanced Genomics Breeding Section, Institute of Crop Science, National Agriculture and Food Research Organization, 1-2 Ohwashi, Tsukuba, Ibaraki, 305-8634, Japan.
Nat Commun ; 10(1): 4033, 2019 09 27.
Article en En | MEDLINE | ID: mdl-31562302
Eukaryotic positive-strand RNA viruses replicate their genomes in membranous compartments formed in a host cell, which sequesters the dsRNA replication intermediate from antiviral immune surveillance. Here, we find that soybean has developed a way to overcome this sequestration. We report the positional cloning of the broad-spectrum soybean mosaic virus resistance gene Rsv4, which encodes an RNase H family protein with dsRNA-degrading activity. An active-site mutant of Rsv4 is incapable of inhibiting virus multiplication and is associated with an active viral RNA polymerase complex in infected cells. These results suggest that Rsv4 enters the viral replication compartment and degrades viral dsRNA. Inspired by this model, we design three plant-gene-derived dsRNases that can inhibit the multiplication of the respective target viruses. These findings suggest a method for developing crops resistant to any target positive-strand RNA virus by fusion of endogenous host genes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Proteínas de Plantas / Glycine max / ARN Polimerasas Dirigidas por ADN / Potyvirus Tipo de estudio: Prognostic_studies Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2019 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Proteínas de Plantas / Glycine max / ARN Polimerasas Dirigidas por ADN / Potyvirus Tipo de estudio: Prognostic_studies Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2019 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido