MicroRNA-3194-3p inhibits metastasis and epithelial-mesenchymal transition of hepatocellular carcinoma by decreasing Wnt/ß-catenin signaling through targeting BCL9.

Yao, Bowen; Li, Yazhao; Wang, Liang; Chen, Tianxiang; Niu, Yongshen; Liu, Qingguang; Liu, Zhikui

Yao, Bowen; Li, Yazhao; Wang, Liang; Chen, Tianxiang; Niu, Yongshen; Liu, Qingguang; Liu, Zhikui.

Afiliación

Yao B; Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
Li Y; Translational Medicine Center, The First Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
Wang L; Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
Chen T; Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
Niu Y; Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
Liu Q; Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.
Liu Z; Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University , Xi'an , China.

Artif Cells Nanomed Biotechnol ; 47(1): 3885-3895, 2019 Dec.

Article en En | MEDLINE | ID: mdl-31561723

RESUMEN

Local and systemic metastasis of hepatocellular carcinoma (HCC) causes the poor prognosis and increasing evidence confirms that aberrant miRNAs were involved in cancer progression. However, the expression and mechanisms of a specific miR-3194-3p in HCC remains unknown. In this research, we demonstrated that miR-3194-3p, significantly down-regulated in HCC tissues and cell lines, was associated with metastasis and recurrence of HCC. Notably, gain- and loss-of-function assays demonstrated that miR-3194-3p inhibited the migration, invasion and epithelial-mesenchymal transition (EMT) of HCC cells in vitro and in vivo. BCL9, up-regulated in HCC tissues, was a direct downstream target of miR-3194-3p and mediated the functional influence of miR-3194-3p. Most importantly, miR-3194-3p exerted its function by regulating ß-catenin pathway. Moreover, miR-3194-3p and BCL9 expression were markedly correlated with adverse clinical features and poor prognosis of HCC patients. We showed that hypoxia was responsible for the down-expression of miR-3194-3p in HCC. Also, the promoting effects of hypoxia on metastasis and EMT of HCC cells were reversed by miR-3194-3p. Altogether, our study suggested that miR-3194-3p inhibits HCC EMT via decreasing Wnt/ß-catenin signaling through targeting BCL9 and might be a therapeutic target for HCC.

Asunto(s)

Carcinoma Hepatocelular/patología; Transición Epitelial-Mesenquimal/genética; Neoplasias Hepáticas/patología; MicroARNs/genética; Factores de Transcripción/genética; Vía de Señalización Wnt/genética; Anciano; Animales; Carcinoma Hepatocelular/genética; Línea Celular Tumoral; Movimiento Celular/genética; Regulación hacia Abajo/genética; Femenino; Regulación Neoplásica de la Expresión Génica; Humanos; Neoplasias Hepáticas/genética; Masculino; Ratones; Persona de Mediana Edad; Invasividad Neoplásica/genética; Metástasis de la Neoplasia/genética

Palabras clave

BCL9; hepatocellular carcinoma; hypoxia; miR-3194-3p; ß-catenin

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Carcinoma Hepatocelular / MicroARNs / Transición Epitelial-Mesenquimal / Vía de Señalización Wnt / Neoplasias Hepáticas Tipo de estudio: Prognostic_studies Límite: Aged / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: Artif Cells Nanomed Biotechnol Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido

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