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Albumin-chaperoned cyanine dye yields superbright NIR-II fluorophore with enhanced pharmacokinetics.
Tian, Rui; Zeng, Qiao; Zhu, Shoujun; Lau, Joseph; Chandra, Swati; Ertsey, Robert; Hettie, Kenneth S; Teraphongphom, Tarn; Hu, Zhubin; Niu, Gang; Kiesewetter, Dale O; Sun, Haitao; Zhang, Xiaodong; Antaris, Alexander L; Brooks, Bernard R; Chen, Xiaoyuan.
Afiliación
  • Tian R; Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health, Bethesda, MD 20892, USA.
  • Zeng Q; Laboratory of Computational Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
  • Zhu S; Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health, Bethesda, MD 20892, USA.
  • Lau J; Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health, Bethesda, MD 20892, USA.
  • Chandra S; Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health, Bethesda, MD 20892, USA.
  • Ertsey R; Department of Otolaryngology, Stanford University, Stanford, CA 94305, USA.
  • Hettie KS; Department of Otolaryngology, Stanford University, Stanford, CA 94305, USA.
  • Teraphongphom T; Department of Otolaryngology, Stanford University, Stanford, CA 94305, USA.
  • Hu Z; State Key Laboratory of Precision Spectroscopy, School of Physics and Materials Science, East China Normal University, Shanghai 200062, P. R. China.
  • Niu G; Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health, Bethesda, MD 20892, USA.
  • Kiesewetter DO; Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health, Bethesda, MD 20892, USA.
  • Sun H; State Key Laboratory of Precision Spectroscopy, School of Physics and Materials Science, East China Normal University, Shanghai 200062, P. R. China.
  • Zhang X; Department of Physics, School of Science, Tianjin University, Tianjin 300354, P. R. China.
  • Antaris AL; Intuitive Surgical, Kifer Rd., Sunnyvale, CA 94086, USA.
  • Brooks BR; Laboratory of Computational Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
  • Chen X; Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health, Bethesda, MD 20892, USA.
Sci Adv ; 5(9): eaaw0672, 2019 09.
Article en En | MEDLINE | ID: mdl-31548981
NIR-II fluorescence imaging greatly reduces scattering coefficients for nearly all tissue types at long wavelengths, benefiting deep tissue imaging. However, most of the NIR-II fluorophores suffer from low quantum yields and/or short circulation time that limit the quality of NIR-II imaging. Here, we engineered a supramolecular assembly of protein complex with lodged cyanine dyes to produce a brilliant NIR-II fluorophore, providing a NIR-II quantum yield of 21.2% with prolonged circulation time. Computational modeling revealed the mechanism for fluorescence enhancement and identified key parameters governing albumin complex for NIR-II fluorophores. Our complex afforded high-resolution microvessel imaging, with a 3-hour imaging window compared to 2 min for free dye alone. Furthermore, the complexation strategy was applied to an antibody-derived assembly, offering high-contrast tumor imaging without affecting the targeting ability of the antibody. This study provides a facile strategy for producing high-performance NIR-II fluorophores by chaperoning cyanine dyes with functional proteins.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Albúmina Sérica Bovina / Medios de Contraste / Imagen Óptica / Colorantes Fluorescentes / Neoplasias Experimentales Límite: Animals Idioma: En Revista: Sci Adv Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Albúmina Sérica Bovina / Medios de Contraste / Imagen Óptica / Colorantes Fluorescentes / Neoplasias Experimentales Límite: Animals Idioma: En Revista: Sci Adv Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos