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Photoswitching enzymatic activity of horseradish peroxidase by graphene oxide for colorimetric immunoassay.
Cao, Genxia; Sun, Dongxue; Gu, Tiantian; Dong, Yuming; Wang, Guang-Li.
Afiliación
  • Cao G; International Joint Research Center for Photoresponsive Molecules and Materials, Key Laboratory of Synthetic and Biological Colloids (Ministry of Education), School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China.
  • Sun D; International Joint Research Center for Photoresponsive Molecules and Materials, Key Laboratory of Synthetic and Biological Colloids (Ministry of Education), School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China.
  • Gu T; International Joint Research Center for Photoresponsive Molecules and Materials, Key Laboratory of Synthetic and Biological Colloids (Ministry of Education), School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China.
  • Dong Y; International Joint Research Center for Photoresponsive Molecules and Materials, Key Laboratory of Synthetic and Biological Colloids (Ministry of Education), School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China.
  • Wang GL; International Joint Research Center for Photoresponsive Molecules and Materials, Key Laboratory of Synthetic and Biological Colloids (Ministry of Education), School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China. Electronic address: glwang@jiangnan.edu.cn.
Biosens Bioelectron ; 145: 111707, 2019 Dec 01.
Article en En | MEDLINE | ID: mdl-31542678
In contrast to the conventional means that the activity of horseradish peroxidase (HRP) is initiated and terminated by the additives of peroxides and strongly acidic stop solutions, this study demonstrates that the enzymatic activity of HRP is switched through the visible light irradiated graphene oxide (GO). And this visible light driven activity of HRP can realize time-precise control without the aids of peroxides (typically H2O2) and acidic stop solutions. The superoxide anions (O2•-) and photogenerated holes (h+) produced by the photo irradiated GO are responsible for activating HRP and the subsequent oxidation of the typical substrates, i.e., 3, 3', 5, 5'-tetramethylbenzidine (TMB) and 2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). It is also validated that the photoswitchable HRP-GO mixture can act as an efficient signal reporter of bioassays by taking the sandwich immunoassay of alpha-fetoprotein (AFP) as an example. The AFP can be detected sensitively and selectively in the linear range from 0.2 fg/mL to 1.0 ng/mL, with a very low detection limit of 0.1 fg/mL. Advantages of the photoswitchable HRP-GO mixture include high catalytic ability, precise time control, and free of additionally harmful reagents.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoensayo / Técnicas Biosensibles / Alfa-Fetoproteínas / Colorimetría Límite: Humans Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunoensayo / Técnicas Biosensibles / Alfa-Fetoproteínas / Colorimetría Límite: Humans Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido