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Evaluation of the neuromuscular junction in a middle-aged mouse model of congenital myasthenic syndrome.
Leite Schetino, Luana Pereira; de Castro Fonseca, Matheus; Magalhães Gomes, Matheus Proença Simão; Costa Valadão, Priscila Aparecida; de Camargo, Wallace Lucio; Rodrigues, Hermann Alecsandro; Andrade, Jéssica Neves; Arantes-Costa, Fernanda Magalhães; Naves, Lígia Araujo; Prado, Carla Máximo; Prado, Vânia Ferreira; Prado, Marco Antônio Máximo; Guatimosim, Cristina.
Afiliación
  • Leite Schetino LP; Faculdade de Medicina, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Minas Gerais, Brazil.
  • de Castro Fonseca M; Laboratório Nacional de Biociências, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, São Paulo, Brazil.
  • Magalhães Gomes MPS; Departamento de Morfologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Costa Valadão PA; Departamento de Morfologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • de Camargo WL; Departamento de Fisiologia e Biofísica, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Rodrigues HA; Departamento de Ciências Básicas da Vida, Instituto de Ciências da Vida, Universidade Federal de Juiz de Fora, Campus Governador Valadares, Minas Gerais, Brazil.
  • Andrade JN; Departamento de Morfologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Arantes-Costa FM; Departamento de Medicina, Escola de Medicina, Universidade de São Paulo, São Paulo, São Paulo, Brazil.
  • Naves LA; Departamento de Fisiologia e Biofísica, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Prado CM; Departmento de Biociências, Universidade Federal de São Paulo, Campus Baixada Santista, São Paulo, Brazil.
  • Prado VF; Robarts Research Institute and Department of Physiology and Pharmacology and Anatomy & Cell Biology, University of Western Ontario, London, Ontario, Canada.
  • Prado MAM; Robarts Research Institute and Department of Physiology and Pharmacology and Anatomy & Cell Biology, University of Western Ontario, London, Ontario, Canada.
  • Guatimosim C; Departamento de Morfologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Muscle Nerve ; 60(6): 790-800, 2019 12.
Article en En | MEDLINE | ID: mdl-31531871
INTRODUCTION: Reduced expression of the vesicular acetylcholine transporter (VAChT) leads to changes in the distribution and shape of synaptic vesicles (SVs) at neuromuscular junctions (NMJs), suggesting vesicular acetylcholine (ACh) as a key component of synaptic structure and function. It is poorly understood how long-term changes in cholinergic transmission contribute to age- and disease-related degeneration in the motor system. METHODS: In this study we performed confocal imaging, electrophysiology, electron microscopy, and analyses of respiratory mechanics of the diaphragm NMJ components in 12-month-old wild-type (WT) and VAChTKDHOM mice. RESULTS: Diaphragms of NMJs of the VAChTKDHOM mice were similar to those in WT mice in number, colocalization, and fragmentation of pre-/postsynaptic components. However, they had increased spontaneous SV exocytosis, miniature endplate potential frequency, and diminished MEPP amplitude. No impairment in respiratory mechanics at rest was observed, probably due to the large neurotransmission safety factor of the diaphragm. DISCUSSION: The present findings help us to understand the consequences of reduced ACh release at the NMJs during aging.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vesículas Sinápticas / Envejecimiento / Diafragma / Síndromes Miasténicos Congénitos / Unión Neuromuscular Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Muscle Nerve Año: 2019 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vesículas Sinápticas / Envejecimiento / Diafragma / Síndromes Miasténicos Congénitos / Unión Neuromuscular Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Muscle Nerve Año: 2019 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos