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The C4 Ppc promoters of many C4 grass species share a common regulatory mechanism for gene expression in the mesophyll cell.
Gupta, Shipan Das; Levey, Myles; Schulze, Stefanie; Karki, Shanta; Emmerling, Jan; Streubel, Monika; Gowik, Udo; Paul Quick, W; Westhoff, Peter.
Afiliación
  • Gupta SD; Institute of Plant Molecular and Developmental Biology, Universitätsstrasse 1, Heinrich-Heine-University, 40225, Düsseldorf, Germany.
  • Levey M; Department of Biotechnology and Genetic Engineering, Noakhali Science and Technology University, Noakahli, 3814, Bangladesh.
  • Schulze S; Institute of Plant Molecular and Developmental Biology, Universitätsstrasse 1, Heinrich-Heine-University, 40225, Düsseldorf, Germany.
  • Karki S; Institute of Plant Molecular and Developmental Biology, Universitätsstrasse 1, Heinrich-Heine-University, 40225, Düsseldorf, Germany.
  • Emmerling J; International Rice Research Institute, Los Banos, Laguna, 4031, Philippines.
  • Streubel M; National Citrus Development Program, Kirtipur, Kathmandu, Nepal.
  • Gowik U; Institute of Plant Molecular and Developmental Biology, Universitätsstrasse 1, Heinrich-Heine-University, 40225, Düsseldorf, Germany.
  • Paul Quick W; Institute of Plant Molecular and Developmental Biology, Universitätsstrasse 1, Heinrich-Heine-University, 40225, Düsseldorf, Germany.
  • Westhoff P; Institute of Plant Molecular and Developmental Biology, Universitätsstrasse 1, Heinrich-Heine-University, 40225, Düsseldorf, Germany.
Plant J ; 101(1): 204-216, 2020 01.
Article en En | MEDLINE | ID: mdl-31529521
C4 photosynthetic plants have evolved from C3 ancestors and are characterized by differential expression of several hundred genes. Strict compartmentalization of key C4 enzymes either to mesophyll (M) or bundle sheath cells is considered a crucial step towards the evolution of C4 photosynthesis. In this study, we demonstrate that the 5'-flanking sequences of the C4 type phosphoenolpyruvate carboxylase (Ppc) gene from three C4 grass species could drive M-cell-specific expression of a reporter gene in rice. In addition to that, we identified about 450 bp (upstream of their transcription start site) of the analyzed C4 Ppc promoters contain all the essential regulatory elements for driving M-cell-specific expression in rice leaves. Importantly, four motifs of conserved nucleotide sequences (CNSs) were also determined, which are essential for the activity of the promoter. A putative interaction between the CNSs and an unknown upstream element(s) is required for driving M-cell-specific expression. This work identifies the evolutionary conservation of C4 Ppc regulatory mechanisms of multiple closely related C4 grass species.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regiones Promotoras Genéticas / Células del Mesófilo Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2020 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regiones Promotoras Genéticas / Células del Mesófilo Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2020 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido