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Characterization of a putative glycoside hydrolase family 43 arabinofuranosidase from Aspergillus niger and its potential use in beer production.
Li, Xiaomin; Xie, Xizhu; Liu, Jun; Wu, Dianhui; Cai, Guolin; Lu, Jian.
Afiliación
  • Li X; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; Jiangsu Provinci
  • Xie X; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China.
  • Liu J; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; Jiangsu Provinci
  • Wu D; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; Jiangsu Provinci
  • Cai G; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; Jiangsu Provinci
  • Lu J; The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; Jiangsu Provinci
Food Chem ; 305: 125382, 2020 Feb 01.
Article en En | MEDLINE | ID: mdl-31525590
During the mashing process for brewing beer, incomplete degradation of arabinoxylan in barley malt may cause an intense filterability problem. The present study cloned a putative arabinofuranosidase (AnAbf), one of the debranching enzymes, from Aspergillus niger, to explore its application for improving filterability. Recombinant AnAbf (rAnAbf) showed activity towards both synthetic and natural substrates, such as 4-nitrophenyl α-l-arabinofuranoside (pNPαAraf) and malt water extractable arabinoxylan (WEAX), which was maximized at a temperature of 50 °C and pH of 5.5. Metal ions did not increase the activity of rAnAbf, indicating a difference in its C-terminal domain from that of type II GH43 family members. rAnAbf also exhibited a synergistic effect with ß-xylanase against WEAX. The filtration rate of the wort increased by 12.8% after supplementing with rAnAbf during the initial stage of mashing. A slight decrease in viscosity and an unexpected increase in turbidity were observed.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aspergillus niger / Cerveza / Xilanos / Glicósido Hidrolasas Idioma: En Revista: Food Chem Año: 2020 Tipo del documento: Article Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aspergillus niger / Cerveza / Xilanos / Glicósido Hidrolasas Idioma: En Revista: Food Chem Año: 2020 Tipo del documento: Article Pais de publicación: Reino Unido