Sensitive enzyme immunoassay of cephalexin residues in milk, hen tissues, and eggs.
J Assoc Off Anal Chem
; 71(5): 915-20, 1988.
Article
en En
| MEDLINE
| ID: mdl-3148610
A sensitive enzyme immunoassay for cephalexin (CEX) was developed using the rabbit antiserum to CEX, beta-D-galactosidase-labeled CEX, and a double-antibody separation method. The immunogen of CEX was prepared by coupling the amino group of CEX to thiol groups introduced into bovine serum albumin by the use of N-(m-maleimidobenzoyloxy)succinimide as a cross-linker. Highly titered antiserum to CEX was produced in rabbits immunized with the immunogen. Enzyme labeling of CEX with beta-D-galactosidase was done by using N-(gamma-maleimidobutyryloxy)succinimide as the cross-linker. The limit of detection was 30 ng CEX/mL sample solution. Application of the method to CEX drug residues detected 30 ng/mL in milk, 60 ng/g in egg yolk, and 400 ng/g in hen tissue.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Residuos de Medicamentos
/
Cefalexina
/
Leche
/
Huevos
/
Carne
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Assoc Off Anal Chem
Año:
1988
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Estados Unidos