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Structure to function analysis with antigenic characterization of a hypothetical protein,HPAG1_0576 from Helicobacter pylori HPAG1.
Ashrafi, Hanan; Siraji, Muntequa Ishtiaq; Showva, Nazmir Nur; Hossain, Md Mozamme; Hossan, Tareq; Hasan, Md Ashraful; Shohael, Abdullah Mohammad; Shawan, Mohammad Mahfuz Ali Khan.
Afiliación
  • Ashrafi H; Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
  • Siraji MI; Department of Biomedicine,University of Bergen, Bergen, Norway.
  • Showva NN; Department of Biomedicine,University of Bergen, Bergen, Norway.
  • Hossain MM; Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka,Bangladesh.
  • Hossan T; Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
  • Hasan MA; Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
  • Shohael AM; Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
  • Shawan MMAK; Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
Bioinformation ; 15(7): 456-466, 2019.
Article en En | MEDLINE | ID: mdl-31485131
Helicobacter pylori, a unique gastric pathogen causing chronic inflammation in the gastric mucosa with a possibility to develop gastric cancer has one-third of its proteins still uncharacterized. In this study, a hypothetical protein (HP) namely HPAG1_0576 from H. pylori HPAG1 was chosen for detailed computational analysis of its structural, functional and epitopic properties. The primary, secondary and 3D structure/model of the selected HP was constructed. Then refinement and structure validation were done, which indicated a good quality of the newly constructed model. ProFunc and STRING suggested that HPAG1_0576 shares 98% identity with a carcinogenic factor, TNF-α inducing protein (Tip-α ) of H. pylori. IEDB immunoinformatics tool predicted VLMLQACTCPNTSQRNS from position 19-35 as most potential B-cell linear epitope and SFLKSKQL from position 5-12 as most potent conformational epitope. Alternatively, FALVRARGF and FLCGLGVLM were predicted as most immunogenic CD8+ and CD4+ T-cell epitopes respectively. At the same time findings of IFN epitope tool suggests that, HPAG1_0576 had a great potential to evoke interferon-gamma (IFN-γ) mediated immune response. However, this experiment is a primary approach for in silico vaccine designing from a HP, findings of this study will provide significant insights in further investigations and will assist in identifying new drug targets/vaccine candidates.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Bioinformation Año: 2019 Tipo del documento: Article País de afiliación: Bangladesh Pais de publicación: Singapur
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Bioinformation Año: 2019 Tipo del documento: Article País de afiliación: Bangladesh Pais de publicación: Singapur