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Tear film substance P: A potential biomarker for diabetic peripheral neuropathy.
Tummanapalli, Shyam Sunder; Willcox, Mark D P; Issar, Tushar; Yan, Aimy; Pisarcikova, Jana; Kwai, Natalie; Poynten, Ann M; Krishnan, Arun V; Markoulli, Maria.
Afiliación
  • Tummanapalli SS; School of Optometry & Vision Science, University of New South Wales, Australia. Electronic address: s.tummanapalli@unsw.edu.au.
  • Willcox MDP; School of Optometry & Vision Science, University of New South Wales, Australia.
  • Issar T; Prince of Wales Clinical School, University of New South Wales, Australia.
  • Yan A; Prince of Wales Clinical School, University of New South Wales, Australia.
  • Pisarcikova J; School of Optometry & Vision Science, University of New South Wales, Australia.
  • Kwai N; Prince of Wales Clinical School, University of New South Wales, Australia.
  • Poynten AM; Department of Endocrinology, Prince of Wales Hospital, Australia.
  • Krishnan AV; Prince of Wales Clinical School, University of New South Wales, Australia.
  • Markoulli M; School of Optometry & Vision Science, University of New South Wales, Australia.
Ocul Surf ; 17(4): 690-698, 2019 10.
Article en En | MEDLINE | ID: mdl-31476517
OBJECTIVE: To explore the changes that occur in the concentrations of substance P (SP) and calcitonin gene-related peptide (CGRP) in tears as a result of corneal denervation and its association with diabetic peripheral neuropathy (DPN). METHODS: Sixty-three individuals with type 1 diabetes/type 2 diabetes (T1D/T2D) and 34 age-matched healthy controls underwent a detailed assessment of neuropathy using the Total Neuropathy Score (TNS). The concentration of SP and CGRP in tears was measured by enzyme-linked immunosorbent assay. The corneal sub-basal nerve plexus was imaged using corneal confocal microscopy. Corneal nerve fibre length, fibre density, branch density, total branch density, nerve fractal dimension and inferior whorl length were quantified. RESULTS: In T1D, the median [IQR] concentration of SP in tears was significantly reduced in those with DPN, (130 [61-692]pg/mL) compared to both control subjects (763 [405-1555]pg/mL, P < 0.01) and in those without DPN (914 [339-1832]pg/mL, P = 0.01); the concentration of CGRP was not changed. In T2D, there was no difference in neuropeptides between participants with diabetes and controls, regardless of neuropathic status. In T1D and T2D, corneal nerve parameters were significantly different between those with DPN or without DPN and controls. A significant correlation was noted between the concentration of tear film SP and TNS in T1D (r = -0.49; P < 0.001) and corneal nerve fibre density (r = 0.45; P < 0.001). The concentration of tear film CGRP was correlated significantly with the reduction of corneal nerve fibre density (r = 0.41; P = 0.01) in T1D. CONCLUSION: Tear film SP may provide a potential non-invasive biomarker for assessing neuropathy in T1D.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lágrimas / Sustancia P / Neuropatías Diabéticas Tipo de estudio: Clinical_trials / Observational_studies / Prevalence_studies / Risk_factors_studies Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Ocul Surf Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lágrimas / Sustancia P / Neuropatías Diabéticas Tipo de estudio: Clinical_trials / Observational_studies / Prevalence_studies / Risk_factors_studies Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Ocul Surf Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos