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Disruption of the Lotus japonicus transporter LjNPF2.9 increases shoot biomass and nitrate content without affecting symbiotic performances.
Sol, Stefano; Valkov, Vladimir Totev; Rogato, Alessandra; Noguero, Mélanie; Gargiulo, Laura; Mele, Giacomo; Lacombe, Benoit; Chiurazzi, Maurizio.
Afiliación
  • Sol S; Institute of Biosciences and Bioresources, IBBR, CNR, Via P. Castellino 111, 80131, Naples, Italy.
  • Valkov VT; Institute of Biosciences and Bioresources, IBBR, CNR, Via P. Castellino 111, 80131, Naples, Italy.
  • Rogato A; Institute of Biosciences and Bioresources, IBBR, CNR, Via P. Castellino 111, 80131, Naples, Italy.
  • Noguero M; BPMP, Univ. Montpellier, CNRS, INRA, SupAgro, Montpellier, France.
  • Gargiulo L; Istituto per i Sistemi Agricoli e Forestali del Mediterraneo, ISAFOM, CNR, Via Patacca 85, 80056, Ercolano, Italy.
  • Mele G; Istituto per i Sistemi Agricoli e Forestali del Mediterraneo, ISAFOM, CNR, Via Patacca 85, 80056, Ercolano, Italy.
  • Lacombe B; BPMP, Univ. Montpellier, CNRS, INRA, SupAgro, Montpellier, France.
  • Chiurazzi M; Institute of Biosciences and Bioresources, IBBR, CNR, Via P. Castellino 111, 80131, Naples, Italy. maurizio.chiurazzi@ibbr.cnr.it.
BMC Plant Biol ; 19(1): 380, 2019 Aug 30.
Article en En | MEDLINE | ID: mdl-31470797
BACKGROUND: After uptake from soil into the root tissue, distribution and allocation of nitrate throughout the whole plant body, is a critical step of nitrogen use efficiency (NUE) and for modulation of plant growth in response to various environmental conditions. In legume plants nitrate distribution is also important for the regulation of the nodulation process that allows to fix atmospheric N (N2) through the symbiotic interaction with rhizobia (symbiotic nitrogen fixation, SNF). RESULTS: Here we report the functional characterization of the Lotus japonicus gene LjNPF2.9, which is expressed mainly in the root vascular structures, a key localization for the control of nitrate allocation throughout the plant body. LjNPF2.9 expression in Xenopus laevis oocytes induces 15NO3 accumulation indicating that it functions as a nitrate importer. The phenotypic characterization of three independent knock out mutants indicates an increased shoot biomass in the mutant backgrounds. This phenotype is associated to an increased/decreased nitrate content detected in the shoots/roots. Furthermore, our analysis indicates that the accumulation of nitrate in the shoot does not affect the nodulation and N-Fixation capacities of the knock out mutants. CONCLUSIONS: This study shows that LjNPF2.9 plays a crucial role in the downward transport of nitrate to roots, occurring likely through a xylem-to-phloem loading-mediated activity. The increase of the shoot biomass and nitrate accumulation might represent a relevant phenotype in the perspective of an improved NUE and this is further reinforced in legume plants by the reported lack of effects on the SNF efficiency.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Transporte de Membrana / Proteínas de Plantas / Simbiosis / Lotus / Nitratos Idioma: En Revista: BMC Plant Biol Asunto de la revista: BOTANICA Año: 2019 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Transporte de Membrana / Proteínas de Plantas / Simbiosis / Lotus / Nitratos Idioma: En Revista: BMC Plant Biol Asunto de la revista: BOTANICA Año: 2019 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido