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A negative feedback loop of H19/miR-675/VDR mediates therapeutic effect of cucurmin in the treatment of glioma.
Pan, Jie-Xiang; Chen, Tu-Nan; Ma, Kang; Wang, Shi; Yang, Chuan-Yan; Cui, Gao-Yu.
Afiliación
  • Pan JX; Department of Neurosurgery, The First Hospital Affiliated to Army Medical University (Southwest Hospital), Chongqing, China.
  • Chen TN; Department of Neurosurgery, The First Hospital Affiliated to Army Medical University (Southwest Hospital), Chongqing, China.
  • Ma K; Department of Neurosurgery, The First Hospital Affiliated to Army Medical University (Southwest Hospital), Chongqing, China.
  • Wang S; Department of Neurosurgery, The First Hospital Affiliated to Army Medical University (Southwest Hospital), Chongqing, China.
  • Yang CY; Department of Neurosurgery, The First Hospital Affiliated to Army Medical University (Southwest Hospital), Chongqing, China.
  • Cui GY; Department of Neurosurgery, The First Hospital Affiliated to Army Medical University (Southwest Hospital), Chongqing, China.
J Cell Physiol ; 235(3): 2171-2182, 2020 03.
Article en En | MEDLINE | ID: mdl-31468534
Curcumin (CUR) shows a remarkable antitumor activity against a wide range of cancers such as glioma, but its underlying mechanism remains elusive. In this study, we aimed to explore the potential role of H19/miR-675/vitamin D receptor (VDR) in the effect of CUR against glioma. Real-time polymerase chain reaction and western-blot analysis were used to study the effect of CUR or 1,25-dihydroxyvitamin D (1,25(OH)2 D3 ) on the expression of H19, miR-675, and VDR. In addition, the effect of H19 on VDR expression was also studied. Furthermore, the expression of H19, miR-675, and VDR between CUR-loaded nanoparticles (NPs) and NP groups was compared, and the interaction among H19, miR-675, and VDR was analyzed by in-silicon and luciferase assays. In a dose-dependent manner, CUR and 1,25(OH)2 D3 both downregulated the expression of H19 and miR-675 but increased the expression of VDR. In addition, H19 evidently reduced the mRNA and protein levels of VDR. Furthermore, VDR was confirmed as a target gene of miR-675, which significantly reduced the expression of VDR. Finally, the administration of CUR evidently decreased tumor volume. CUR-loaded NP group exhibited lower levels of H19 and miR-675, while the NP group showed higher levels of VDR mRNA and protein. In summary, it is the first time that the involvement of a negative feedback loop of H19/miR-675/VDR has been demonstrated in the development of glioma. Therefore, H19 might serve as a new biomarker for the diagnosis and treatment of glioma.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores de Calcitriol / Curcumina / MicroARNs / ARN Largo no Codificante / Glioma Límite: Animals / Female / Humans Idioma: En Revista: J Cell Physiol Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores de Calcitriol / Curcumina / MicroARNs / ARN Largo no Codificante / Glioma Límite: Animals / Female / Humans Idioma: En Revista: J Cell Physiol Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos