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Verifying measurements of residual calcium content in demineralised cortical bone.
Ariffin, A F; Yusof, N; Mohd, S; Rahman, S A; Ramalingam, S; Mansor, A; Min, N G.
Afiliación
  • Ariffin AF; Bone Bank, National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
  • Yusof N; Bone Bank, National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
  • Mohd S; Bone Bank, National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia. suhailimohd@ummc.edu.my.
  • Rahman SA; Waste and Environmental Technology Division, Malaysian Nuclear Agency, 43000, Bangi, Kajang, Selangor, Malaysia.
  • Ramalingam S; Bone Bank, National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
  • Mansor A; Bone Bank, National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
  • Min NG; Bone Bank, National Orthopaedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Cell Tissue Bank ; 20(4): 527-534, 2019 Dec.
Article en En | MEDLINE | ID: mdl-31456097
Calcium contents of demineralised human cortical bone determined by titrimetric assay and atomic absorption spectrophotometry technique were verified by comparing to neutron activation analysis which has high recovery of more than 90%. Conversion factors determined from the comparison is necessary to correct the calcium content for each technique. Femurs from cadaveric donors were cut into cortical rings and demineralised in 0.5 M hydrochloric acid for varying immersion times. Initial calcium content in the cortical bone measured by titration was 4.57%, only 21% of the measurement by neutron activation analysis; while measured by atomic absorption spectrophotometer was 13.4%, only 61% of neutron activation analysis. By comparing more readings with the measurements by neutron activation analysis with 93% recovery, a conversion factor of 4.83 was verified and applied for the readings by titration and 1.45 for atomic absorption spectrophotometer in calculating the correct calcium contents. The residual calcium content started to reduce after the cortical bone was demineralised in hydrochloric acid for 8 h and reduced to 13% after 24 h. Using the linear relationship, the residual calcium content could be reduced to less than 8% after immersion in hydrochloric acid for 40 h. Atomic absorption spectrophotometry technique is the method of choice for calcium content determination as it is more reliable compared to titrimetric assay.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcio / Fémur / Hueso Cortical Límite: Adult / Humans / Male / Middle aged Idioma: En Revista: Cell Tissue Bank Asunto de la revista: HISTOLOGIA / TRANSPLANTE Año: 2019 Tipo del documento: Article País de afiliación: Malasia Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcio / Fémur / Hueso Cortical Límite: Adult / Humans / Male / Middle aged Idioma: En Revista: Cell Tissue Bank Asunto de la revista: HISTOLOGIA / TRANSPLANTE Año: 2019 Tipo del documento: Article País de afiliación: Malasia Pais de publicación: Países Bajos