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Aggregation-induced emission fluorescent probe for monitoring endogenous alkaline phosphatase in living cells.
Li, Yaqian; Xie, Ruihua; Pang, Xiao; Zhou, Zile; Xu, Hai; Gu, Biao; Wu, Cuiyan; Li, Haitao; Zhang, Youyu.
Afiliación
  • Li Y; Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, PR China.
  • Xie R; Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, PR China.
  • Pang X; Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, PR China.
  • Zhou Z; Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, PR China.
  • Xu H; Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, PR China.
  • Gu B; Key Laboratory of Functional Organometallic Materials of College of Hunan Province, College of Chemistry and Materials Science, Hengyang Normal University, Hengyang, 421008, PR China.
  • Wu C; Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, PR China.
  • Li H; Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, PR China. Electronic address: haitao-li@hunnu.edu.cn.
  • Zhang Y; Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, PR China.
Talanta ; 205: 120143, 2019 Dec 01.
Article en En | MEDLINE | ID: mdl-31450444
Alkaline phosphatase (ALP) is a non-specific phosphate monoesterase and often regarded as an important biomarker of hypothyroidism and hepatobiliary diseases in medical diagnosis. In-situ detection of endogenous ALP and exploration of the distribution of ALP in cells are of great importance for the diagnosis of diseases associated with ALP. In this work, we designed and synthesized an aggregation-induced emission (AIE) fluorescent probe, (E)-2-(((9H-fluoren-9-ylidene) hydrazono)methyl)phenyl dihydrogen phosphate (FAS-P), that can respond to ALP with a remarkable large Stokes shift (>200 nm) based on excited state intramolecular proton transfer (ESIPT) mechanism. The probe FAS-P has high selectivity and sensitivity to the detection of ALP. And there is a linear relationship between the fluorescence intensity of FAS-P and ALP activity in the range of 1-100 U L-1, the limit of detection (LOD) is as low as 0.6 U L-1. More importantly, we successfully applied FAS-P to detect ALP in living cells and the monitoring of ALP in real time.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatasa Alcalina / Pruebas de Enzimas / Colorantes Fluorescentes Límite: Humans Idioma: En Revista: Talanta Año: 2019 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatasa Alcalina / Pruebas de Enzimas / Colorantes Fluorescentes Límite: Humans Idioma: En Revista: Talanta Año: 2019 Tipo del documento: Article Pais de publicación: Países Bajos