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Amidrazone modified acrylic fabric activated with cyanuric chloride: A novel and efficient support for horseradish peroxidase immobilization and phenol removal.
Almulaiky, Yaaser Q; El-Shishtawy, Reda M; Aldhahri, Musab; Mohamed, Saleh A; Afifi, Mohamed; Abdulaal, Wesam H; Mahyoub, Jazem A.
Afiliación
  • Almulaiky YQ; Chemistry Department, Faculty of Sciences and Arts, University of Jeddah, Khulais, P.O. Box 355, Khulais 21921, Saudi Arabia; Chemistry Department, Faculty of Applied Science, Taiz University, Taiz, Yemen. Electronic address: yaseralmoliki@hotmail.com.
  • El-Shishtawy RM; Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, P. O. Box 80200, Jeddah 21589, Saudi Arabia; Dyeing, Printing and Textile Auxiliaries Department, Textile Research Division, National Research Center, Dokki, 71516, Cairo, Egypt.
  • Aldhahri M; Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, P. O. Box 80200, Jeddah 21589, Saudi Arabia; Center of Nanotechnology, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
  • Mohamed SA; Molecular Biology Department, National Research Centre, Cairo, Egypt.
  • Afifi M; Department of Biochemistry, Faculty of Science, University of Jeddah, P.O. Box 80203, Jeddah 21589, Saudi Arabia; Biochemistry Department, Faculty of Veterinary Medicine, Zagazig University, Egypt.
  • Abdulaal WH; Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, P. O. Box 80200, Jeddah 21589, Saudi Arabia.
  • Mahyoub JA; Department of Biological Sciences, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia.
Int J Biol Macromol ; 140: 949-958, 2019 Nov 01.
Article en En | MEDLINE | ID: mdl-31445147
In this study, hydrazine treated acrylic fabrics (polyacrylonitrile, PAN) activated with cyanuric chloride was developed as supporting material for horseradish peroxidase (HRP) immobilization. The immobilization of HRP onto the modified supporting material was achieved after being end-over-end incubated for 12 h. Field emission scanning electron microscopy and Fourier-transform infrared spectroscopy techniques were used to confirm the successful immobilization. Reusability experiment was performed to estimate the ability of the immobilized HRP to recover the reaction medium, in which it was observed to retain 78% of its original activity after 10 cycles. Relative to the soluble HRP, the optimum pH and temperature for the immobilized HRP were shifted to 7-7.5 and 50 °C, respectively. The kinetic parameters of guaiacol and H2O2 for the immobilized HRP were determined to be Km/Vmax = 57.61, 11.35 and Kcat/Km = 1.87, 1.86, respectively, while the values for the free form were Km/Vmax = 41.49, 6.23 and Kcat/Km = 1.87, 1.86, respectively. Compared to the soluble form, the immobilized HRP exhibited higher resistance toward metal ions and some organic solvents. For an application perspective. The immobilization of HRP using this procedure has the potential to be used for industrial application and wastewater treatment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Triazinas / Resinas Acrílicas / Fenol / Enzimas Inmovilizadas / Peroxidasa de Rábano Silvestre Idioma: En Revista: Int J Biol Macromol Año: 2019 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Triazinas / Resinas Acrílicas / Fenol / Enzimas Inmovilizadas / Peroxidasa de Rábano Silvestre Idioma: En Revista: Int J Biol Macromol Año: 2019 Tipo del documento: Article Pais de publicación: Países Bajos