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The Zea mays mutants opaque2 and opaque16 disclose lysine change in waxy maize as revealed by RNA-Seq.
Wang, Wei; Niu, Suzhen; Dai, Yi; Wang, Mingchun; Li, Yan; Yang, Wenpeng; Zhao, Degang.
Afiliación
  • Wang W; The State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou University, Guiyang, 550025, China.
  • Niu S; Guizhou Institute of Upland Food Crops, Guiyang Station for DUS Testing Center of New Plant Varieties (MOA), Guizhou Academy of Agricultural Sciences, Guiyang, 550006, China.
  • Dai Y; The State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou University, Guiyang, 550025, China.
  • Wang M; The State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou University, Guiyang, 550025, China.
  • Li Y; Guizhou Institute of Upland Food Crops, Guiyang Station for DUS Testing Center of New Plant Varieties (MOA), Guizhou Academy of Agricultural Sciences, Guiyang, 550006, China.
  • Yang W; The State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou University, Guiyang, 550025, China.
  • Zhao D; Guizhou Institute of Upland Food Crops, Guiyang Station for DUS Testing Center of New Plant Varieties (MOA), Guizhou Academy of Agricultural Sciences, Guiyang, 550006, China. ywpmaize@126.com.
Sci Rep ; 9(1): 12265, 2019 08 22.
Article en En | MEDLINE | ID: mdl-31439855
In maize, opaque2 (o2) and opaque16 (o16) alleles can increase lysine content, while the waxy (wx) gene can enhance the amylopectin content of grains. In our study, o2 and o16 alleles were backcrossed into waxy maize line (wxwx). The o2o2o16o16wxwx lines had amylopectin contents similar to those of waxy line. Their nutritional value was better than waxy line, but the mechanism by which the o2 and o16 alleles increased the lysine content of waxy maize remained unclear. The o2o2o16o16wxwx lines and their parents on kernels (18th day after pollination) were subjected to RNA sequencing (RNA-Seq). The RNA-Seq analysis revealed 272 differentially expressed genes (DEGs). Functional analyses revealed that these DEGs were mainly related to biomass metabolism. Among them, in o2o2o16o16wxwx lines, 15 genes encoding α-zein were down-regulated, which resulted in the reduction of α-zein synthesis and increased lysine content; lkr/sdh1 and Zm00001d020984.1 genes involved in the lysine degradation pathway were down-regulated, thereby inhibited lysine degradation; sh2, bt2 and ae1 genes involved in starch metabolism were upregulated, leaded to wrinkling kernel and farinaceous endosperm. Our transcriptional-level identification of key genes responsible for increased grain lysine content and farinaceous endosperm formation following introgression of o2 and o16 alleles should promote molecular breeding for maize quality.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Factores de Transcripción / Regulación de la Expresión Génica de las Plantas / Zea mays / Proteínas de Unión al ADN / Alelos / RNA-Seq / Amilopectina Tipo de estudio: Prognostic_studies Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Factores de Transcripción / Regulación de la Expresión Génica de las Plantas / Zea mays / Proteínas de Unión al ADN / Alelos / RNA-Seq / Amilopectina Tipo de estudio: Prognostic_studies Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido