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Anti-EpCAM antibodies for detection of metastatic carcinoma in effusions and peritoneal wash.
Carneiro, Fabiana Pirani; Muniz-Junqueira, Maria Imaculada; De Vasconcelos Carneiro, Marcos; De Araújo Oliveira, Ísis; Soares, Aluízio Carlos; De Vargas Haar, Nathália; Takano, Gustavo Henrique Soares; De Sousa Vianna, Leonora Maciel; De Carvalho Caldas, Guilherme; Vieira, Danillo Leal Marinho; Frutuoso, Lígia Lins; Brito, Larissa Matos Rodrigues; De Siqueira, Rafael Vieira Martins; Parente, Amanda Moreira; De Castro, Tercia Maria Mendes Lousa; Peres, Isabela; Mendes, Lianna Martha Soares; Dos Santos Borges, Tatiana Karla; Ferreira, Vânia Moraes; Motoyama, Andrea Barretto.
Afiliación
  • Carneiro FP; Pathological Anatomy Center of University Hospital of Brasília, Brasília 70840-050, Brazil.
  • Muniz-Junqueira MI; Pathology Department of Brasília University, Brasília 70910-900, Brazil.
  • De Vasconcelos Carneiro M; Catholic University of Brasilia, Brasília 71966-700, Brazil.
  • De Araújo Oliveira Í; Pathology Department of Brasília University, Brasília 70910-900, Brazil.
  • Soares AC; Catholic University of Brasilia, Brasília 71966-700, Brazil.
  • De Vargas Haar N; Pathological Anatomy Center of University Hospital of Brasília, Brasília 70840-050, Brazil.
  • Takano GHS; Pathology Department of Brasília University, Brasília 70910-900, Brazil.
  • De Sousa Vianna LM; Pathology Department of Brasília University, Brasília 70910-900, Brazil.
  • De Carvalho Caldas G; Pathological Anatomy Center of University Hospital of Brasília, Brasília 70840-050, Brazil.
  • Vieira DLM; Pathological Anatomy Center of University Hospital of Brasília, Brasília 70840-050, Brazil.
  • Frutuoso LL; Pathological Anatomy Center of University Hospital of Brasília, Brasília 70840-050, Brazil.
  • Brito LMR; Pathological Anatomy Center of University Hospital of Brasília, Brasília 70840-050, Brazil.
  • De Siqueira RVM; Pathological Anatomy Center of University Hospital of Brasília, Brasília 70840-050, Brazil.
  • Parente AM; Pathological Anatomy Center of University Hospital of Brasília, Brasília 70840-050, Brazil.
  • De Castro TMML; Pathological Anatomy Center of University Hospital of Brasília, Brasília 70840-050, Brazil.
  • Peres I; Pathological Anatomy Center of University Hospital of Brasília, Brasília 70840-050, Brazil.
  • Mendes LMS; Pathology Department of Brasília University, Brasília 70910-900, Brazil.
  • Dos Santos Borges TK; Pathological Anatomy Center of University Hospital of Brasília, Brasília 70840-050, Brazil.
  • Ferreira VM; Pathological Anatomy Center of University Hospital of Brasília, Brasília 70840-050, Brazil.
  • Motoyama AB; Pathology Department of Brasília University, Brasília 70910-900, Brazil.
Oncol Lett ; 18(2): 2019-2024, 2019 Aug.
Article en En | MEDLINE | ID: mdl-31423273
Epithelial cell adhesion molecule (EpCAM) has been used as diagnostic/prognostic marker and therapeutic target. The aim of the present study was to compare immunoreactivity of antibodies against distinct epitopes in the ectodomain of EpCAM for detection of carcinoma from different primary sites and of different histological types in effusions and peritoneal wash. Two antibodies against epitopes in the EGF-like domain I (clones Moc-31 and Ber-EP4) and one antibody against the epitope in the cysteine-poor region (158210) of EpCAM were used (all commercially available). Independently of the clone used, EpCAM overexpression was observed in almost all samples when all the adenocarcinoma samples were analyzed together. By using Moc-31, EpCAM overexpression was observed in all samples of adenocarcinoma. Absence of EpCAM overexpression was observed in a few adenocarcinoma samples at some sites of tumor origin, including ovary, breast and stomach, when Ber-EP4 and 158210 were used. Regarding carcinomas aside from adenocarcinomas, histological types, such as squamous cell, urothelial and small cell carcinoma showed different degrees of EpCAM expression according to the antibody used. In squamous cell carcinoma, overexpression was observed only with the clone 158210. It was concluded that, overall, most samples of metastatic carcinoma from effusions showed overexpression of EpCAM. However, there are significant variations in its detection according to the primary site, histological type of the carcinoma and depending on the antibody used. Thus, the use of more than one type of anti-EpCAM antibody would increase the chance of its detection in metastatic carcinoma effusion.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Oncol Lett Año: 2019 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Grecia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Oncol Lett Año: 2019 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Grecia