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Lanthanide-dependent methanol dehydrogenase from the legume symbiotic nitrogen-fixing bacterium Bradyrhizobium diazoefficiens strain USDA110.
Wang, Lun; Suganuma, Soya; Hibino, Ayumi; Mitsui, Ryoji; Tani, Akio; Matsumoto, Takashi; Ebihara, Akio; Fitriyanto, Nanung Agus; Pertiwiningrum, Ambar; Shimada, Masaya; Hayakawa, Takashi; Nakagawa, Tomoyuki.
Afiliación
  • Wang L; The United Graduate School of Agricultural Science, Gifu University, Gifu, Japan.
  • Suganuma S; Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
  • Hibino A; Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
  • Mitsui R; Department of Biochemistry, Faculty of Science, Okayama University of Science, Kita-ku, Okayama, Japan.
  • Tani A; Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama, Japan.
  • Matsumoto T; Application Laboratories, Rigaku Corporation, Tokyo, Japan.
  • Ebihara A; The United Graduate School of Agricultural Science, Gifu University, Gifu, Japan; Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
  • Fitriyanto NA; Faculty of Animal Science, Universitas Gadjah Mada, Karangmalang, Yogyakarta, 55281, Indonesia.
  • Pertiwiningrum A; Faculty of Animal Science, Universitas Gadjah Mada, Karangmalang, Yogyakarta, 55281, Indonesia.
  • Shimada M; The United Graduate School of Agricultural Science, Gifu University, Gifu, Japan; Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
  • Hayakawa T; The United Graduate School of Agricultural Science, Gifu University, Gifu, Japan; Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
  • Nakagawa T; The United Graduate School of Agricultural Science, Gifu University, Gifu, Japan; Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan. Electronic address: t_nakaga@gifu-u.ac.jp.
Enzyme Microb Technol ; 130: 109371, 2019 Nov.
Article en En | MEDLINE | ID: mdl-31421721
The legume symbiotic nitrogen-fixing bacterium, B. diazoefficiens strain USDA110, utilizes methanol for growth in the presence of light lanthanides, such as La3+, Ce3+, Pr3+ or Nd3+, and its cells possess significant methanol dehydrogenase (MDH) activity. We purified MDH to homogeneity from B. diazoefficiens strain USDA110 grown in a methanol/Ce3+ medium; the protein was identified as XoxF5-type MDH (blr6213). The purified XoxF contained 0.58 cerium atoms per enzyme subunit. Moreover, the in-solution structure of XoxF was analyzed by small angle X-ray scattering (SAXS) analysis; the radius of gyration (Rg) and maximum particle dimension (Dmax) of XoxF were calculated to be 32.3 and 96.8 Å, respectively, suggesting that XoxF adopts a dimer structure in solution. These results show that B. diazoefficiens strain USDA110 has XoxF, a lanthanides-dependent MDH, which has methanol oxidation activity and is induced by methanol/lanthanaides, and that lanthanide is one of the important factors in methanol utilization by the strain.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bradyrhizobium / Elementos de la Serie de los Lantanoides / Oxidorreductasas de Alcohol Tipo de estudio: Prognostic_studies Idioma: En Revista: Enzyme Microb Technol Año: 2019 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bradyrhizobium / Elementos de la Serie de los Lantanoides / Oxidorreductasas de Alcohol Tipo de estudio: Prognostic_studies Idioma: En Revista: Enzyme Microb Technol Año: 2019 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos