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The role of chitin-rich skeletal organic matrix on the crystallization of calcium carbonate in the crustose coralline alga Leptophytum foecundum.
Rahman, M Azizur; Halfar, Jochen; Adey, Walter H; Nash, Merinda; Paulo, Carlos; Dittrich, Maria.
Afiliación
  • Rahman MA; Department of Chemical & Physical Sciences, University of Toronto at Mississauga, Toronto, Canada. aziz@climatechangeresearch.ca.
  • Halfar J; Department of Physical and Environmental Sciences, University of Toronto Scarborough, Toronto, Canada. aziz@climatechangeresearch.ca.
  • Adey WH; Department of Chemical & Physical Sciences, University of Toronto at Mississauga, Toronto, Canada.
  • Nash M; Department of Botany, Smithsonian Institution, Washington, DC, 20560, USA.
  • Paulo C; Research School of Earth Sciences, Australian National University, Canberra, Australia.
  • Dittrich M; Department of Physical and Environmental Sciences, University of Toronto Scarborough, Toronto, Canada.
Sci Rep ; 9(1): 11869, 2019 08 15.
Article en En | MEDLINE | ID: mdl-31417166
The organic matrix (OM) contained in marine calcifiers has a key role in the regulation of crystal deposition, such as crystalline structure, initiation of mineralization, inhibition, and biological/environmental control. However, the functional properties of the chitin-rich skeletal organic matrix on the biological aspect of crystallization in crustose coralline algae have not yet been investigated. Hence, the characterization of organic matrices in the biomineralization process of this species was studied to understand the functions of these key components for structural formation and mineralization of calcium carbonate crystals. We purified skeletal organic matrix proteins from this species and explored how these components are involved in the mineralization of calcium carbonate crystals and environmental control. Intriguingly, the analytical investigation of the skeletal OM revealed the presence of chitin in the crustose coralline alga Leptophytum foecundum. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the OM revealed a high molecular mass protein as 300-kDa. Analysis of glycosylation activity exposed two strong glycoproteins as 300-kDa and 240-kDa. Our study of the biominerals of live collected specimens found that in addition to Mg-calcite up to 30% aragonite were present in the skeleton. Our experiment demonstrated that the chitin-rich skeletal OM of coralline algae plays a key role in the biocalcification process by enabling the formation of Mg-calcite. In addition, this OM did not inhibit the formation of aragonite suggesting there is an as yet unidentified process in the living coralline that prevents the formation of aragonite in the living skeletal cell walls.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carbonato de Calcio / Quitina / Rhodophyta Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carbonato de Calcio / Quitina / Rhodophyta Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido