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The interactome of KRAB zinc finger proteins reveals the evolutionary history of their functional diversification.
Helleboid, Pierre-Yves; Heusel, Moritz; Duc, Julien; Piot, Cécile; Thorball, Christian W; Coluccio, Andrea; Pontis, Julien; Imbeault, Michaël; Turelli, Priscilla; Aebersold, Ruedi; Trono, Didier.
Afiliación
  • Helleboid PY; School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
  • Heusel M; Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
  • Duc J; School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
  • Piot C; School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
  • Thorball CW; School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
  • Coluccio A; School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
  • Pontis J; School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
  • Imbeault M; School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
  • Turelli P; School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
  • Aebersold R; Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.
  • Trono D; Faculty of Science, University of Zurich, Zurich, Switzerland.
EMBO J ; 38(18): e101220, 2019 09 16.
Article en En | MEDLINE | ID: mdl-31403225
Krüppel-associated box (KRAB)-containing zinc finger proteins (KZFPs) are encoded in the hundreds by the genomes of higher vertebrates, and many act with the heterochromatin-inducing KAP1 as repressors of transposable elements (TEs) during early embryogenesis. Yet, their widespread expression in adult tissues and enrichment at other genetic loci indicate additional roles. Here, we characterized the protein interactome of 101 of the ~350 human KZFPs. Consistent with their targeting of TEs, most KZFPs conserved up to placental mammals essentially recruit KAP1 and associated effectors. In contrast, a subset of more ancient KZFPs rather interacts with factors related to functions such as genome architecture or RNA processing. Nevertheless, KZFPs from coelacanth, our most distant KZFP-encoding relative, bind the cognate KAP1. These results support a hypothetical model whereby KZFPs first emerged as TE-controlling repressors, were continuously renewed by turnover of their hosts' TE loads, and occasionally produced derivatives that escaped this evolutionary flushing by development and exaptation of novel functions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Proteínas Represoras / Proteína 28 que Contiene Motivos Tripartito Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans / Pregnancy Idioma: En Revista: EMBO J Año: 2019 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Proteínas Represoras / Proteína 28 que Contiene Motivos Tripartito Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans / Pregnancy Idioma: En Revista: EMBO J Año: 2019 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido