In Vivo and In Vitro Analysis of IL-10 in the NZB Leukemic Model.
Cancer Genomics Proteomics
; 1(5-6): 407-418, 2004.
Article
en En
| MEDLINE
| ID: mdl-31394632
BACKGROUND: The B-1 malignancy, CLL has been associated with a failure to undergo apoptosis and increased endogenous IL-10 production. This study was undertaken to identify IL-10 effects in the NZB murine model of CLL. MATERIALS AND METHODS: Antisense IL-10 was employed in vitro and in vivo to decrease IL-10 protein. Following treatment, cells were analyzed for alterations in cell cycle and RNA was studied for alterations in gene expression. Additional in vivo studies employed NZB mice in which the IL-10 gene was deleted. RESULTS: IL-10 (-/-) knockout NZB mice overwhelmingly failed to develop leukemia. In vitro antisense IL-10 treatment resulted in a G2/M block and apoptosis and in vivo treatment with antisense IL-10 increased the survival of mice. Microarray analysis indicated a significant role for IL-10 in cell cycle regulation via cdc25C up-regulation and decreased p47phox redox activity. CONCLUSION: In summary, IL-10 is a critical survival factor for malignant B cells via anti-apoptotic and cell cycle effects.
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Colección:
01-internacional
Base de datos:
MEDLINE
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Cancer Genomics Proteomics
Asunto de la revista:
BIOQUIMICA
/
GENETICA MEDICA
/
NEOPLASIAS
Año:
2004
Tipo del documento:
Article
Pais de publicación:
Grecia