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Bcl-2/Bax ratio increase does not prevent apoptosis of glia and granular neurons in patients with temporal lobe epilepsy.
Toscano, Eliana C de Brito; Vieira, Érica L M; Portela, Ana C D C; Reis, Joice L J; Caliari, Marcelo V; Giannetti, Alexandre V; Gonçalves, Ana P; Siqueira, José M; Suemoto, Claudia K; Leite, Renata E P; Nitrini, Ricardo; Teixeira, Antônio L; Rachid, Milene A.
Afiliación
  • Toscano ECB; Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Vieira ÉLM; Neuroscience Division, Interdisciplinary Laboratory of Medical Investigation, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Portela ACDC; Neuroscience Division, Interdisciplinary Laboratory of Medical Investigation, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Reis JLJ; Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Caliari MV; Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Giannetti AV; Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Gonçalves AP; Hospital das Clínicas, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Siqueira JM; Hospital das Clínicas, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Suemoto CK; Nuclei of Advanced Epilepsy Treatment - Felício Rocho Hospital, Belo Horizonte, Brazil.
  • Leite REP; Nuclei of Advanced Epilepsy Treatment - Felício Rocho Hospital, Belo Horizonte, Brazil.
  • Nitrini R; Laboratório de Fisiopatologia no Envelhecimento, Universidade de São Paulo, São Paulo, Brazil.
  • Teixeira AL; Laboratório de Fisiopatologia no Envelhecimento, Universidade de São Paulo, São Paulo, Brazil.
  • Rachid MA; Laboratório de Fisiopatologia no Envelhecimento, Universidade de São Paulo, São Paulo, Brazil.
Neuropathology ; 39(5): 348-357, 2019 Oct.
Article en En | MEDLINE | ID: mdl-31392787
Temporal lobe epilepsy (TLE) is usually associated with hippocampal sclerosis (HS), characterized by gliosis and neuronal loss, mainly in the cornus ammonis (CA). Regardless the type of HS, gliosis is associated with neuronal loss. Indeed, glial reactivation seems to induce both neuronal and glial apoptosis. Anti-apoptotic mechanisms are also activated in order to contain the cell death in chronic epilepsy. However, the role of the intrinsic apoptosis pathway in human TLE is unclear, mainly in relation to glial death. The purpose of this study was to evaluate the reactive gliosis areas in parallel with Bcl-2/Bax ratio and active caspase 3 immunoreactivity in hippocampi of TLE patients in comparison with control hippocampi. We also sought to investigate whether the levels of these markers were correlated with TLE clinical parameters. Paraffin-embedded sclerotic and control hippocampi were collected for immunohistochemical analyses of glial fibrillary acidic protein (GFAP), human leucocyte antigen DR (HLA-DR), neuronal nuclei protein (NeuN), Bax, Bcl-2 and active caspase 3. Sclerotic hippocampi presented higher immunoreactivity areas of GFAP and HLA-DR than controls, with similar values in HS types 1 and 2. Bcl-2 protein expression was increased in epileptic hippocampi, while Bax expression was similar to controls. Despite Bcl2/Bax ratio increase, granular neurons and glia exhibited active caspase 3 expression in TLE hippocampi, while controls did not show staining for the same marker. In conclusion, glial and neuronal death is increased in sclerotic hippocampi, independently of HS type, and co-localized with gliosis. Furthermore, Bcl-2/Bax ratio increase does not prevent expression of active caspase 3 by glia and granular neurons in TLE.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neuroglía / Proteínas Proto-Oncogénicas c-bcl-2 / Epilepsia del Lóbulo Temporal / Proteína X Asociada a bcl-2 / Neuronas Límite: Adolescent / Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Neuropathology Asunto de la revista: NEUROLOGIA / PATOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Australia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neuroglía / Proteínas Proto-Oncogénicas c-bcl-2 / Epilepsia del Lóbulo Temporal / Proteína X Asociada a bcl-2 / Neuronas Límite: Adolescent / Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Neuropathology Asunto de la revista: NEUROLOGIA / PATOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Australia