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Melatonin may prevent or reverse polycystic ovary syndrome in rats.
Lombardi, Leonardo Augusto; Mattos, Leandro Sabará de; Simões, Ricardo Santos; Florencio-Silva, Rinaldo; Sasso, Gisela Rodrigues da Silva; Carbonel, Adriana Aparecida Ferraz; Simões, Manuel Jesus; Baracat, Edmund Chada; Soares, José Maria.
Afiliación
  • Lombardi LA; Departamento de Morfologia e Genética, Escola Paulista de Medicina da Universidade Federal de São Paulo, EPM/Unifesp, São Paulo, SP, Brasil.
  • Mattos LS; Departamento de Morfologia e Genética, Escola Paulista de Medicina da Universidade Federal de São Paulo, EPM/Unifesp, São Paulo, SP, Brasil.
  • Simões RS; Departamento de Obstetrícia e Ginecologia, Faculdade de Medicina da Universidade de São Paulo, FMUSP, São Paulo, SP, Brasil.
  • Florencio-Silva R; Departamento de Morfologia e Genética, Escola Paulista de Medicina da Universidade Federal de São Paulo, EPM/Unifesp, São Paulo, SP, Brasil.
  • Sasso GRDS; Departamento de Morfologia e Genética, Escola Paulista de Medicina da Universidade Federal de São Paulo, EPM/Unifesp, São Paulo, SP, Brasil.
  • Carbonel AAF; Departamento de Morfologia e Genética, Escola Paulista de Medicina da Universidade Federal de São Paulo, EPM/Unifesp, São Paulo, SP, Brasil.
  • Simões MJ; Departamento de Morfologia e Genética, Escola Paulista de Medicina da Universidade Federal de São Paulo, EPM/Unifesp, São Paulo, SP, Brasil.
  • Baracat EC; Departamento de Obstetrícia e Ginecologia, Faculdade de Medicina da Universidade de São Paulo, FMUSP, São Paulo, SP, Brasil.
  • Soares JM; Departamento de Obstetrícia e Ginecologia, Faculdade de Medicina da Universidade de São Paulo, FMUSP, São Paulo, SP, Brasil.
Rev Assoc Med Bras (1992) ; 65(7): 1008-1014, 2019 Aug 05.
Article en En | MEDLINE | ID: mdl-31389515
OBJECTIVE: To evaluate the ovarian effects of melatonin (Mel) in a rat model of polycystic-ovary-syndrome (PCOS) before and after permanent estrus induction. METHODS: Thirty-two adult-female rats with regular estrous cycle were equally divided into four groups: 1) GCtrl - at estrous phase. 2) GPCOS - at permanent-estrous phase. 3) GMel1 - treated for 60 days with Mel (0.4 mg/Kg) during permanent estrus induction and 4) GMel2 - rats with PCOS and treated for 60 days with Mel. After that, the animals were euthanized, and the ovaries were removed and processed for paraffin embedding. Sections were stained with H.E. for histomorphometry or subjected to immunohistochemistry for Ki-67 and cleaved caspase-3 (Casp-3) detections. RESULTS: The GPCOS showed lack of corpus luteum and several ovarian cysts, as well as interstitial-like cells. The presence of corpus luteum and a significant increase in primary and antral follicles were observed in Mel-treated groups, which also showed a decrease in the number of ovarian cysts and in the area occupied by interstitial-like cells. These results were more evident in GMel1. The percentage of Ki-67-positive cells was significantly higher in the Mel-treated groups, mainly in the GMel2, as compared to GPCOS. On the other hand, the percentage of Casp-3-positive cells was significantly lower in granulosa cells of GMel1, whereas it was significantly higher in the interstitial-like cells of GMel2, in comparison to GPCOS. CONCLUSION: Melatonin administration prevents the permanent estrus state in the PCOS rat model. This effect is more efficient when melatonin is administered before permanent estrus induction.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Síndrome del Ovario Poliquístico / Melatonina Tipo de estudio: Evaluation_studies / Observational_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Rev Assoc Med Bras (1992) Año: 2019 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Síndrome del Ovario Poliquístico / Melatonina Tipo de estudio: Evaluation_studies / Observational_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Rev Assoc Med Bras (1992) Año: 2019 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil