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The Genomic Landscape of Centromeres in Cancers.
Saha, Anjan K; Mourad, Mohamad; Kaplan, Mark H; Chefetz, Ilana; Malek, Sami N; Buckanovich, Ronald; Markovitz, David M; Contreras-Galindo, Rafael.
Afiliación
  • Saha AK; Medical Scientist Training Program, University of Michigan, Ann Arbor, MI, 48109, USA.
  • Mourad M; Program in Cancer Biology, University of Michigan, Ann Arbor, MI, 48109, USA.
  • Kaplan MH; Department of Internal Medicine, University of Michigan, Ann Arbor, MI, 48109, USA.
  • Chefetz I; Department of Internal Medicine, University of Michigan, Ann Arbor, MI, 48109, USA.
  • Malek SN; Department of Internal Medicine, University of Michigan, Ann Arbor, MI, 48109, USA.
  • Buckanovich R; The Hormel Institute, University of Minnesota, Austin, MN, 55912, USA.
  • Markovitz DM; Department of Internal Medicine, University of Michigan, Ann Arbor, MI, 48109, USA.
  • Contreras-Galindo R; Magee-Womens Research Institute, University of Pittsburgh, Pittsburgh, PA, 15213, USA.
Sci Rep ; 9(1): 11259, 2019 08 02.
Article en En | MEDLINE | ID: mdl-31375789
Centromere genomics remain poorly characterized in cancer, due to technologic limitations in sequencing and bioinformatics methodologies that make high-resolution delineation of centromeric loci difficult to achieve. We here leverage a highly specific and targeted rapid PCR methodology to quantitatively assess the genomic landscape of centromeres in cancer cell lines and primary tissue. PCR-based profiling of centromeres revealed widespread heterogeneity of centromeric and pericentromeric sequences in cancer cells and tissues as compared to healthy counterparts. Quantitative reductions in centromeric core and pericentromeric markers (α-satellite units and HERV-K copies) were observed in neoplastic samples as compared to healthy counterparts. Subsequent phylogenetic analysis of a pericentromeric endogenous retrovirus amplified by PCR revealed possible gene conversion events occurring at numerous pericentromeric loci in the setting of malignancy. Our findings collectively represent a more comprehensive evaluation of centromere genetics in the setting of malignancy, providing valuable insight into the evolution and reshuffling of centromeric sequences in cancer development and progression.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biomarcadores de Tumor / Centrómero / Evolución Molecular / Carcinogénesis / Neoplasias Límite: Humans Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biomarcadores de Tumor / Centrómero / Evolución Molecular / Carcinogénesis / Neoplasias Límite: Humans Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido