PAWH1 and PAWH2 are plant-specific components of an Arabidopsis endoplasmic reticulum-associated degradation complex.

Lin, Liangguang; Zhang, Congcong; Chen, Yongwu; Wang, Yi; Wang, Dinghe; Liu, Xiaolei; Wang, Muyang; Mao, Juan; Zhang, Jianjun; Xing, Weiman; Liu, Linchuan; Li, Jianming

Lin, Liangguang; Zhang, Congcong; Chen, Yongwu; Wang, Yi; Wang, Dinghe; Liu, Xiaolei; Wang, Muyang; Mao, Juan; Zhang, Jianjun; Xing, Weiman; Liu, Linchuan; Li, Jianming.

Afiliación

Lin L; Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, 201602, Shanghai, China.
Zhang C; University of Chinese Academy of Sciences, 100004, Beijing, China.
Chen Y; Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, 201602, Shanghai, China.
Wang Y; University of Chinese Academy of Sciences, 100004, Beijing, China.
Wang D; Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, 201602, Shanghai, China.
Liu X; University of Chinese Academy of Sciences, 100004, Beijing, China.
Wang M; Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, 201602, Shanghai, China.
Mao J; University of Chinese Academy of Sciences, 100004, Beijing, China.
Zhang J; Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, 201602, Shanghai, China.
Xing W; Shanghai Institute of Plant Physiology and Ecology, The Center of Excellence for Molecular Plant Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China.
Liu L; Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, 201602, Shanghai, China.
Li J; Shanghai Institute of Plant Physiology and Ecology, The Center of Excellence for Molecular Plant Sciences, Chinese Academy of Sciences, 300 Fenglin Road, 200032, Shanghai, China.

Nat Commun ; 10(1): 3492, 2019 08 02.

Article en En | MEDLINE | ID: mdl-31375683

RESUMEN

Endoplasmic reticulum-associated degradation (ERAD) is a unique mechanism to degrade misfolded proteins via complexes containing several highly-conserved ER-anchored ubiquitin ligases such as HMG-CoA reductase degradation1 (Hrd1). Arabidopsis has a similar Hrd1-containing ERAD machinery; however, our knowledge of this complex is limited. Here we report two closely-related Arabidopsis proteins, Protein Associated With Hrd1-1 (PAWH1) and PAWH2, which share a conserved domain with yeast Altered Inheritance of Mitochondria24. PAWH1 and PAWH2 localize to the ER membrane and associate with Hrd1 via EMS-mutagenized Bri1 Suppressor7 (EBS7), a plant-specific component of the Hrd1 complex. Simultaneously elimination of two PAWHs constitutively activates the unfolded protein response and compromises stress tolerance. Importantly, the pawh1 pawh2 double mutation reduces the protein abundance of EBS7 and Hrd1 and inhibits degradation of several ERAD substrates. Our study not only discovers additional plant-specific components of the Arabidopsis Hrd1 complex but also reveals a distinct mechanism for regulating the Hrd1 stability.

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Risk_factors_studies Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido

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