Acute unfolding of a single protein immediately stimulates recruitment of ubiquitin protein ligase E3C (UBE3C) to 26S proteasomes.

Gottlieb, Colin D; Thompson, Airlia C S; Ordureau, Alban; Harper, J Wade; Kopito, Ron R

Gottlieb, Colin D; Thompson, Airlia C S; Ordureau, Alban; Harper, J Wade; Kopito, Ron R.

Afiliación

Gottlieb CD; Department of Biology, Stanford University, Stanford, California 94305.
Thompson ACS; Department of Biology, Stanford University, Stanford, California 94305.
Ordureau A; Department of Cell Biology, Blavatnik Institute of Harvard Medical School, Boston, Massachusetts 02115.
Harper JW; Department of Cell Biology, Blavatnik Institute of Harvard Medical School, Boston, Massachusetts 02115.
Kopito RR; Department of Biology, Stanford University, Stanford, California 94305 kopito@stanford.edu.

J Biol Chem ; 294(45): 16511-16524, 2019 11 08.

Article en En | MEDLINE | ID: mdl-31375563

RESUMEN

The intracellular accumulation of aggregated misfolded proteins is a cytopathological hallmark of neurodegenerative diseases. However, the functional relationship between protein misfolding or aggregation and the cellular proteostasis network that monitors and maintains proteome health is poorly understood. Previous studies have associated translational suppression and transcriptional remodeling with the appearance of protein aggregates, but whether these responses are induced by aggregates or their misfolded monomeric or oligomeric precursors remains unclear. Because aggregation in cells is rapid, nonlinear, and asynchronous, it has not been possible to deconvolve these kinetically linked processes to determine the earliest cellular responses to misfolded proteins. Upon removal of the synthetic, biologically inert ligand shield-1 (S1), AgDD, an engineered variant FK506-binding protein (FKBP1A), rapidly (t½ â¼5 min) unfolds and self-associates, forming detergent-insoluble, microscopic cytoplasmic aggregates. Using global diglycine-capture (K-GG) proteomics, we found here that this solubility transition is associated with immediate increases in ubiquitylation of AgDD itself, along with that of endogenous proteins that are components of the ribosome and the 26S proteasome. We also found that the earliest cellular responses to acute S1 removal include recruitment of ubiquitin protein ligase E3C (UBE3C) to the 26S proteasome and ubiquitylation of two key proteasomal ubiquitin receptors, 26S proteasome regulatory subunit RPN10 (RPN10) and Rpn13 homolog (RPN13 or ADRM1). We conclude that these proteasomal responses are due to AgDD protein misfolding and not to the presence of detergent-insoluble aggregates.

Asunto(s)

Complejo de la Endopetidasa Proteasomal/metabolismo; Ubiquitina-Proteína Ligasas/metabolismo; Células HEK293; Humanos; Péptidos y Proteínas de Señalización Intracelular/metabolismo; Morfolinas/química; Morfolinas/metabolismo; Agregado de Proteínas; Subunidades de Proteína/metabolismo; Desplegamiento Proteico; Proteómica; Proteostasis; Interferencia de ARN; ARN Interferente Pequeño/metabolismo; Proteínas de Unión a Tacrolimus/genética; Proteínas de Unión a Tacrolimus/metabolismo; Ubiquitina-Proteína Ligasas/antagonistas & inhibidores; Ubiquitina-Proteína Ligasas/genética; Ubiquitinación

Palabras clave

ADRM1; E3 ubiquitin ligase; RPN13; UBE3C; proteasome; protein aggregation; protein misfolding; ubiquitin

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ubiquitina-Proteína Ligasas / Complejo de la Endopetidasa Proteasomal Límite: Humans Idioma: En Revista: J Biol Chem Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos

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