Ultrasensitive Detection of Amyloid-ß Using Cellular Prion Protein on the Highly Conductive Au Nanoparticles-Poly(3,4-ethylene dioxythiophene)-Poly(thiophene-3-acetic acid) Composite Electrode.
Anal Chem
; 91(17): 11259-11265, 2019 09 03.
Article
en En
| MEDLINE
| ID: mdl-31373200
A highly sensitive electrochemical impedance sensor for amyloid beta oligomer (AßO) was fabricated using a cellular prion protein (PrPC) bioreceptor linked with poly(thiophene-3-acetic acid) transducer. An additional thin layer of poly(3,4-ethylene dioxythiophene) embedded with gold nanoparticles was employed to provide high electrical conductivity and a large surface area. The sensing performace was investigated in terms of sensitivity and detection range. The fabricated sensor exhibited extremely low detection limit at a subfemtomolar level with a wide detection range from 10-8 to 104 nM and its utility was established in mice infected with Alzheimer's disease (AD). The developed AßO sensor could be utilized for early diagnosis of AD.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polímeros
/
Tiofenos
/
Péptidos beta-Amiloides
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Compuestos Bicíclicos Heterocíclicos con Puentes
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Enfermedad de Alzheimer
/
Proteínas Priónicas
/
Acetatos
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Animals
Idioma:
En
Revista:
Anal Chem
Año:
2019
Tipo del documento:
Article
Pais de publicación:
Estados Unidos