Effects of Docosahexaenoic Acid on Chemokine Expression in Human Conjunctival Fibroblasts.
Curr Eye Res
; 45(1): 81-86, 2020 01.
Article
en En
| MEDLINE
| ID: mdl-31364439
Purpose: We assessed the production of chemokines by human conjunctival fibroblasts in response to inflammation and the effects of omega (ω)-3 fatty acids on chemokine expression.Methods: Primary cultures of human conjunctival fibroblasts were incubated with interleukin-4 (IL-4) and tumor necrosis factor-alpha (TNF-α). The expression of eotaxin-1 and RANTES in response to pretreatment with docosahexaenoic acid (DHA) was investigated. Moreover, western blotting was used to evaluate the effects of DHA on the activation of nuclear factor (NF)-κB and signal transducer and activator of transcription 6 (STAT6).Results: The expression of eotaxin-1 mRNA was significantly suppressed by pretreatment with DHA with IL-4 and TNF-α costimulation. RANTES expression was similarly suppressed, but the difference was not significant. The secretion of eotaxin-1 and RANTES was significantly lower in DHA-pretreated cells than in vehicle-treated cells. Western blotting for NF-κB and STAT6 showed that these proteins were downregulated in the DHA pretreatment group compared with those in the vehicle control group.Conclusion: The results of this study suggested that DHA could have applications in the management of allergic inflammation.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Conjuntivitis Alérgica
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Ácidos Docosahexaenoicos
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Regulación de la Expresión Génica
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Conjuntiva
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Quimiocina CCL5
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Quimiocina CCL11
Límite:
Humans
Idioma:
En
Revista:
Curr Eye Res
Año:
2020
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Reino Unido