Enriching for Low-Abundance Serum Proteins Using ProteoMiner™ and Protein-Level HPLC.
Methods Mol Biol
; 2024: 103-117, 2019.
Article
en En
| MEDLINE
| ID: mdl-31364045
The mammalian immune system acts to protect the body from harmful diseases ranging from cancer to infection. Differentially expressed proteins as a result of such an immune response can shed light on the mechanism of disease or serve as biomarkers. These biomarkers can be used in a diagnostic capacity or as correlates of protection following vaccination. Protein levels in the circulatory system are considered representative of the system as a whole, making serum an ideal matrix for surveilling immune responses. However, serum proteomics using mass spectrometry is extremely challenging due to the complexity of the matrix and the dynamic range of protein concentration. This chapter will describe two orthogonal enrichment strategies that can be used sequentially or in isolation to improve the identification of low-abundance serum proteins by mass spectrometry.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biomarcadores
/
Cromatografía Líquida de Alta Presión
/
Proteómica
Límite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2019
Tipo del documento:
Article
País de afiliación:
Canadá
Pais de publicación:
Estados Unidos