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Oxidized Cell-Free DNA Role in the Antioxidant Defense Mechanisms under Stress.
Filev, A D; Shmarina, G V; Ershova, E S; Veiko, N N; Martynov, A V; Borzikova, M A; Poletkina, A A; Dolgikh, O A; Veiko, V P; Bekker, A A; Chirkov, A V; Volynshchikov, Z N; Deviataikina, A S; Shashin, D M; Puretskiy, V K; Tabakov, V J; Izhevskaya, V L; Kutsev, S I; Kostyuk, S V; Umriukhin, P E.
Afiliación
  • Filev AD; Research Centre for Medical Genetics (RCMG), Moskvorechie 1, Moscow 115478, Russia.
  • Shmarina GV; Research Centre for Medical Genetics (RCMG), Moskvorechie 1, Moscow 115478, Russia.
  • Ershova ES; I.M. Sechenov First Moscow State Medical University (Sechenov University), Mohovaya str., 11 str.5, 125007 Moscow, Russia.
  • Veiko NN; G.N. Gabrichevsky Institute of Epidemiology and Microbiology, Admiral Makarov str. 10, 125367 Moscow, Russia.
  • Martynov AV; Research Centre for Medical Genetics (RCMG), Moskvorechie 1, Moscow 115478, Russia.
  • Borzikova MA; I.M. Sechenov First Moscow State Medical University (Sechenov University), Mohovaya str., 11 str.5, 125007 Moscow, Russia.
  • Poletkina AA; Research Centre for Medical Genetics (RCMG), Moskvorechie 1, Moscow 115478, Russia.
  • Dolgikh OA; Research Centre for Medical Genetics (RCMG), Moskvorechie 1, Moscow 115478, Russia.
  • Veiko VP; Research Centre for Medical Genetics (RCMG), Moskvorechie 1, Moscow 115478, Russia.
  • Bekker AA; I.M. Sechenov First Moscow State Medical University (Sechenov University), Mohovaya str., 11 str.5, 125007 Moscow, Russia.
  • Chirkov AV; Research Centre for Medical Genetics (RCMG), Moskvorechie 1, Moscow 115478, Russia.
  • Volynshchikov ZN; Research Centre for Medical Genetics (RCMG), Moskvorechie 1, Moscow 115478, Russia.
  • Deviataikina AS; Research Centre for Medical Genetics (RCMG), Moskvorechie 1, Moscow 115478, Russia.
  • Shashin DM; A.N. Bach Institute of Biochemistry, bld 33-2, Leninsly Prospect, Moscow 119071, Russia.
  • Puretskiy VK; I.M. Sechenov First Moscow State Medical University (Sechenov University), Mohovaya str., 11 str.5, 125007 Moscow, Russia.
  • Tabakov VJ; I.M. Sechenov First Moscow State Medical University (Sechenov University), Mohovaya str., 11 str.5, 125007 Moscow, Russia.
  • Izhevskaya VL; I.M. Sechenov First Moscow State Medical University (Sechenov University), Mohovaya str., 11 str.5, 125007 Moscow, Russia.
  • Kutsev SI; I.M. Sechenov First Moscow State Medical University (Sechenov University), Mohovaya str., 11 str.5, 125007 Moscow, Russia.
  • Kostyuk SV; I.M. Sechenov First Moscow State Medical University (Sechenov University), Mohovaya str., 11 str.5, 125007 Moscow, Russia.
  • Umriukhin PE; I.M. Sechenov First Moscow State Medical University (Sechenov University), Mohovaya str., 11 str.5, 125007 Moscow, Russia.
Oxid Med Cell Longev ; 2019: 1245749, 2019.
Article en En | MEDLINE | ID: mdl-31360293
The present study focuses on the investigation of the oxidized cell-free DNA (cfDNA) properties in several experimental models, including cultured cerebellum cells, peripheral blood lymphocytes (PBL), plasma, and hippocampus under an acute and chronic unpredictable stress model in rats. Firstly, our study shows that Spectrum Green fluorescence-labeled oxidized cfDNA fragments were transferred into the cytoplasm of 80% of the cerebellum culture cells; meanwhile, the nonoxidized cfDNA fragments do not pass into the cells. Oxidized cfDNA stimulates the antioxidant mechanisms and induction of transcription factor NRF2 expression, followed by an activation of NRF2 signaling pathway genes-rise of Nrf2 and Hmox1 gene expression and consequently NRF2 protein synthesis. Secondly, we showed that stress increases plasma cfDNA concentration in rats corresponding with the duration of the stress exposure. At the same time, our study did not reveal any significant changes of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) level in PBL of rats under acute or chronic stress, probably due to the significantly increased Nrf2 expression, that we found in such conditions. 8-oxodG is one of the most reliable markers of DNA oxidation. We also found an increased level of 8-oxodG in the hippocampal homogenates and hippocampal dentate gyrus in rats subjected to acute and chronic stress. Taken together, our data shows that oxidized cfDNA may play a significant role in systemic and neuronal physiological mechanisms of stress and adaptation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Oxidativo / Ácidos Nucleicos Libres de Células / Antioxidantes Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Oxid Med Cell Longev Asunto de la revista: METABOLISMO Año: 2019 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Estados Unidos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Oxidativo / Ácidos Nucleicos Libres de Células / Antioxidantes Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Oxid Med Cell Longev Asunto de la revista: METABOLISMO Año: 2019 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Estados Unidos