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Robust biodegradation of naproxen and diclofenac by laccase immobilized using electrospun nanofibers with enhanced stability and reusability.
Zdarta, Jakub; Jankowska, Katarzyna; Wyszowska, Marta; Kijenska-Gawronska, Ewa; Zgola-Grzeskowiak, Agnieszka; Pinelo, Manuel; Meyer, Anne S; Moszynski, Dariusz; Jesionowski, Teofil.
Afiliación
  • Zdarta J; Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland. Electronic address: jakub.zdarta@put.poznan.pl.
  • Jankowska K; Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland.
  • Wyszowska M; Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland.
  • Kijenska-Gawronska E; Faculty of Materials Science and Engineering, Warsaw University of Technology, Woloska 141, PL-02507 Warsaw, Poland.
  • Zgola-Grzeskowiak A; Institute of Chemistry and Electrochemistry, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland.
  • Pinelo M; Department of Chemical and Biochemical Engineering, DTU Chemical Engineering, Technical University of Denmark, Soltofts Plads 227, DK-2800 Kgs. Lyngby, Denmark.
  • Meyer AS; Department of Biotechnology and Biomedicine, DTU Bioengineering, Technical University of Denmark, Soltofts Plads 229, DK-2800 Kgs. Lyngby, Denmark.
  • Moszynski D; West Pomeranian University of Technology, Szczecin, Faculty of Chemical Technology and Engineering, Institute of Inorganic Chemical Technology and Environment Engineering, Pulaskiego 10, PL-70322 Szczecin, Poland.
  • Jesionowski T; Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland. Electronic address: teofil.jesionowski@put.poznan.pl.
Mater Sci Eng C Mater Biol Appl ; 103: 109789, 2019 Oct.
Article en En | MEDLINE | ID: mdl-31349507
Enzymatic biodegradation of pharmaceuticals, using enzymes such as laccase, is a green solution for the removal of toxic pollutants that has attracted growing interest over recent years. Moreover, the application of immobilized biocatalysts is relevant for industrial applications, due to the improved stability and reusability of the immobilized enzymes. Thus, in the present study, laccase was immobilized by adsorption and encapsulation using poly(l-lactic acid)-co-poly(ε-caprolactone) (PLCL) electrospun nanofibers as a tailor-made support. The produced biocatalytic systems were applied in the biodegradation of two commonly used anti-inflammatories, naproxen and diclofenac, which are present in wastewaters at environmentally relevant concentrations. The results showed that under optimal process conditions (temperature 25 °C, pH 5 and 3 for naproxen and diclofenac respectively), even from a solution at a concentration of 1 mg L-1, over 90% of both pharmaceuticals was removed by encapsulated laccase in batch mode. Both immobilized enzymes also exhibited high reusability: after five reaction cycles approximately 60% and 40% of naproxen and diclofenac were removed by encapsulated and adsorbed laccase respectively. In addition, a thorough analysis was made of the products of biodegradation of the two studied pollutants. Furthermore, toxicity study of the mixture after biodegradation of the pharmaceuticals showed that the solutions obtained after the process were approximately 65% less toxic than the initial naproxen and diclofenac solutions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biodegradación Ambiental / Diclofenaco / Naproxeno / Lacasa / Nanofibras Límite: Animals Idioma: En Revista: Mater Sci Eng C Mater Biol Appl Año: 2019 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biodegradación Ambiental / Diclofenaco / Naproxeno / Lacasa / Nanofibras Límite: Animals Idioma: En Revista: Mater Sci Eng C Mater Biol Appl Año: 2019 Tipo del documento: Article Pais de publicación: Países Bajos