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Impacts of Deepwater Horizon Crude Oil on Mahi-Mahi (Coryphaena hippurus) Heart Cell Function.
Heuer, Rachael M; Galli, Gina L J; Shiels, Holly A; Fieber, Lynne A; Cox, Georgina K; Mager, Edward M; Stieglitz, John D; Benetti, Daniel D; Grosell, Martin; Crossley Ii, Dane A.
Afiliación
  • Heuer RM; Department of Biological Sciences , University of North Texas , 1511 W. Sycamore Street , Denton Texas 76203 , United States.
  • Galli GLJ; Department of Marine Biology and Ecology , University of Miami Rosenstiel School of Marine and Atmospheric Science , 4600 Rickenbacker Causeway , Miami , Florida 33149 , United States.
  • Shiels HA; University of Manchester, Faculty of Biology, Medicine and Health Sciences , The University of Manchester, Core Technology Facility , Grafton Street , Manchester M13 9PL , U.K.
  • Fieber LA; University of Manchester, Faculty of Biology, Medicine and Health Sciences , The University of Manchester, Core Technology Facility , Grafton Street , Manchester M13 9PL , U.K.
  • Cox GK; Department of Marine Biology and Ecology , University of Miami Rosenstiel School of Marine and Atmospheric Science , 4600 Rickenbacker Causeway , Miami , Florida 33149 , United States.
  • Mager EM; Department of Marine Biology and Ecology , University of Miami Rosenstiel School of Marine and Atmospheric Science , 4600 Rickenbacker Causeway , Miami , Florida 33149 , United States.
  • Stieglitz JD; Department of Integrative Biology , University of Guelph , 50 Stone Road East , Guelph , Ontario Canada , N1G 2W1.
  • Benetti DD; Department of Biological Sciences , University of North Texas , 1511 W. Sycamore Street , Denton Texas 76203 , United States.
  • Grosell M; Department of Marine Biology and Ecology , University of Miami Rosenstiel School of Marine and Atmospheric Science , 4600 Rickenbacker Causeway , Miami , Florida 33149 , United States.
  • Crossley Ii DA; Department of Marine Ecosystems and Society , University of Miami Rosenstiel School of Marine and Atmospheric Science , 4600 Rickenbacker Causeway , Miami , Florida 33149 , United States.
Environ Sci Technol ; 53(16): 9895-9904, 2019 Aug 20.
Article en En | MEDLINE | ID: mdl-31343865
Deepwater Horizon crude oil is comprised of polycyclic aromatic hydrocarbons that cause a number of cardiotoxic effects in marine fishes across all levels of biological organization and at different life stages. Although cardiotoxic impacts have been widely reported, the mechanisms underlying these impairments in adult fish remain understudied. In this study, we examined the impacts of crude oil on cardiomyocyte contractility and electrophysiological parameters in freshly isolated ventricular cardiomyocytes from adult mahi-mahi (Coryphaena hippurus). Cardiomyocytes directly exposed to oil exhibited reduced contractility over a range of environmentally relevant concentrations (2.8-12.9 µg l-1∑PAH). This reduction in contractility was most pronounced at higher stimulation frequencies, corresponding to the upper limits of previously measured in situ mahi heart rates. To better understand the mechanisms underlying impaired contractile function, electrophysiological studies were performed, which revealed oil exposure prolonged cardiomyocyte action potentials and disrupted potassium cycling (9.9-30.4 µg l-1∑PAH). This study is the first to measure cellular contractility in oil-exposed cardiomyocytes from a pelagic fish. Results from this study contribute to previously observed impairments to heart function and whole-animal exercise performance in mahi, underscoring the advantages of using an integrative approach in examining mechanisms of oil-induced cardiotoxicity in marine fish.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hidrocarburos Policíclicos Aromáticos / Contaminantes Químicos del Agua / Perciformes / Petróleo / Contaminación por Petróleo Límite: Animals Idioma: En Revista: Environ Sci Technol Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hidrocarburos Policíclicos Aromáticos / Contaminantes Químicos del Agua / Perciformes / Petróleo / Contaminación por Petróleo Límite: Animals Idioma: En Revista: Environ Sci Technol Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos