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Topogenic signals in integral membrane proteins.
von Heijne, G; Gavel, Y.
Afiliación
  • von Heijne G; Department of Molecular Biology, Karolinska Institutet, Huddinge, Sweden.
Eur J Biochem ; 174(4): 671-8, 1988 Jul 01.
Article en En | MEDLINE | ID: mdl-3134198
Integral membrane proteins are characterized by long apolar segments that cross the lipid bilayer. Polar domains flanking these apolar segments have a more balanced amino acid composition, typical for soluble proteins. We show that the apolar segments from three different kinds of membrane-assembly signals do not differ significantly in amino acid content, but that the inside/outside location of the polar domains correlates strongly with their content of arginyl and lysyl residues, not only for bacterial inner-membrane proteins, but also for eukaryotic.proteins from the endoplasmic reticulum, the plasma membrane, the inner mitochondrial membrane, and the chloroplast thylakoid membrane. A positive-inside rule thus seems to apply universally to all integral membrane proteins, with apolar regions targeting for membrane integration and charged residues providing the topological information.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aminoácidos / Proteínas de la Membrana Idioma: En Revista: Eur J Biochem Año: 1988 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Reino Unido
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aminoácidos / Proteínas de la Membrana Idioma: En Revista: Eur J Biochem Año: 1988 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Reino Unido