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The influence of cationic dendrimers on antibacterial activity of phage endolysin against P. aeruginosa cells.
Ciepluch, Karol; Maciejewska, Barbara; Galczynska, Katarzyna; Kuc-Ciepluch, Dorota; Bryszewska, Maria; Appelhans, Dietmar; Drulis-Kawa, Zuzanna; Arabski, Michal.
Afiliación
  • Ciepluch K; Department of Biochemistry and Genetics, Jan Kochanowski University, Kielce, Poland. Electronic address: karol.ciepluch@ujk.edu.pl.
  • Maciejewska B; Department of Pathogen Biology and Immunology, Institute of Genetics and Microbiology, University of Wroclaw, Wroclaw, Poland.
  • Galczynska K; Department of Biochemistry and Genetics, Jan Kochanowski University, Kielce, Poland.
  • Kuc-Ciepluch D; Department of Biochemistry and Genetics, Jan Kochanowski University, Kielce, Poland.
  • Bryszewska M; Department of General Biophysics, University of Lodz, Lodz, Poland.
  • Appelhans D; Leibniz Institute of Polymer Research Dresden, Dresden, Germany.
  • Drulis-Kawa Z; Department of Pathogen Biology and Immunology, Institute of Genetics and Microbiology, University of Wroclaw, Wroclaw, Poland.
  • Arabski M; Department of Biochemistry and Genetics, Jan Kochanowski University, Kielce, Poland.
Bioorg Chem ; 91: 103121, 2019 10.
Article en En | MEDLINE | ID: mdl-31310881
Nowadays, the researchers make a big effort to find new alternatives to overcome bacterial drug resistance. One option is the application of bacteriophage endolysins enable to degrade peptidoglycan (PG) what in consequence leads to bacterial cell lysis. In this study we examine phage KP27 endolysin mixed with poly(propyleneimine) dendrimers to evaluate an antimicrobial effect against Pseudomonas aeruginosa. Polycationic compounds destabilize bacterial outer membrane (OM) helping endolysins to gain access to PG. We found out that not only bacterial lipopolysaccharide (LPS) is the main hindrance for highly charged cationic dendrimers to disrupt OM and make endolysin reaching the target but also the dendrimer surface modification. The reduction of a positive charge and concentration in maltose poly(propyleneimine) dendrimers significantly increased an antibacterial effect of endolysin. The application of recombinant lysins against Gram-negative bacteria is one of the future therapy options, thus OM permeabilizers such as cationic dendrimers may be of high interest to be combined with PG-degrading enzymes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Endopeptidasas / Pseudomonas aeruginosa / Dendrímeros / Antibacterianos Idioma: En Revista: Bioorg Chem Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Endopeptidasas / Pseudomonas aeruginosa / Dendrímeros / Antibacterianos Idioma: En Revista: Bioorg Chem Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos