Your browser doesn't support javascript.
loading
Circulating tumour cell RNA characterisation from colorectal cancer patient blood after inertial microfluidic enrichment.
Winter, Marnie; Cai, Zhen; Winkler, Katharina; Georgiou, Kristen; Inglis, Daniel; Lavranos, Tina; Rezaei, Meysam; Warkiani, Majid; Thierry, Benjamin.
Afiliación
  • Winter M; Future Industries Institute and ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of South Australia, Mawson Lakes Campus, Mawson Lakes, South Australia, Australia.
  • Cai Z; Laboratory Medicine Center, NanFang Hospital, Southern Medical University, Guangzhou, China.
  • Winkler K; Future Industries Institute and ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of South Australia, Mawson Lakes Campus, Mawson Lakes, South Australia, Australia.
  • Georgiou K; Bionomics, Thebarton, South Australia, Australia.
  • Inglis D; Bionomics, Thebarton, South Australia, Australia.
  • Lavranos T; Bionomics, Thebarton, South Australia, Australia.
  • Rezaei M; Future Industries Institute and ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of South Australia, Mawson Lakes Campus, Mawson Lakes, South Australia, Australia.
  • Warkiani M; School of Biomedical Engineering, University of Technology Sydney, Sydney, Ultimo, NSW, Australia.
  • Thierry B; Institute of Molecular Medicine, Sechenov First Moscow State University, Moscow, Russia.
MethodsX ; 6: 1512-1520, 2019.
Article en En | MEDLINE | ID: mdl-31304099
The detection and molecular analysis of circulating tumour cells (CTCs) potentially provides a significant insight to the characterisation of disease, stage of progression and therapeutic options for cancer patients. Following on from the protocol by Warkiani et al. 2016, which describes a method of enriching CTCs from cancer patient blood with inertial microfluidics, we describe a method to measure the CTC RNA expression in the enriched fraction using droplet digital PCR and compare transcript detection with and without RNA pre-amplification. •Inertial microfluidics combined with droplet digital PCR is advantageous as it allows for CTC enrichment and subsequent RNA analysis from patient blood. This allows for patient tumour analysis with increased sensitivity and precision compared to quantitative Real Time PCR and enables the direct quantification of nucleic acids without the need for tumour biopsy.•This method demonstrates an efficient approach providing important insights into the analysis of colorectal cancer patients' CTCs using a specific gene subset or biomarkers, an approach that may be tailored to tumour type or expanded to larger panels.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Guideline Idioma: En Revista: MethodsX Año: 2019 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Guideline Idioma: En Revista: MethodsX Año: 2019 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Países Bajos