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Antagonistic Interactions between Benzo[a]pyrene and Fullerene (C60) in Toxicological Response of Marine Mussels.
Barranger, Audrey; Langan, Laura M; Sharma, Vikram; Rance, Graham A; Aminot, Yann; Weston, Nicola J; Akcha, Farida; Moore, Michael N; Arlt, Volker M; Khlobystov, Andrei N; Readman, James W; Jha, Awadhesh N.
Afiliación
  • Barranger A; School of Biological and Marine Sciences, University of Plymouth, Plymouth PL4 8AA, UK.
  • Langan LM; School of Biological and Marine Sciences, University of Plymouth, Plymouth PL4 8AA, UK.
  • Sharma V; School of Biomedical Sciences, University of Plymouth, Plymouth PL4 8AA, UK.
  • Rance GA; School of Chemistry, University of Nottingham, University Park, Nottingham NG7 2RD, UK.
  • Aminot Y; Nanoscale and Microscale Research Centre, University of Nottingham, University Park, Nottingham NG7 2RD, UK.
  • Weston NJ; Centre for Chemical Sciences, University of Plymouth, Plymouth PL4 8AA, UK.
  • Akcha F; Nanoscale and Microscale Research Centre, University of Nottingham, University Park, Nottingham NG7 2RD, UK.
  • Moore MN; Ifremer, Laboratory of Ecotoxicology, F-44311, CEDEX 03 Nantes, France.
  • Arlt VM; School of Biological and Marine Sciences, University of Plymouth, Plymouth PL4 8AA, UK.
  • Khlobystov AN; Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth PL1 3HD, UK.
  • Readman JW; European Centre for Environment & Human Health (ECEHH), University of Exeter Medical School, Knowledge Spa, Royal Cornwall Hospital, Cornwall TR1 3LJ, UK.
  • Jha AN; Department of Analytical, Environmental and Forensic Sciences, King's College London, MRC-PHE Centre for Environmental & Health, London SE1 9NH, UK.
Nanomaterials (Basel) ; 9(7)2019 Jul 08.
Article en En | MEDLINE | ID: mdl-31288459
This study aimed to assess the ecotoxicological effects of the interaction of fullerene (C60) and benzo[a]pyrene (B[a]P) on the marine mussel, Mytilus galloprovincialis. The uptake of nC60, B[a]P and mixtures of nC60 and B[a]P into tissues was confirmed by Gas Chromatography-Mass Spectrometry (GC-MS), Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) and Inductively Coupled Plasma Mass Spectrometer (ICP-MS). Biomarkers of DNA damage as well as proteomics analysis were applied to unravel the interactive effect of B[a]P and C60. Antagonistic responses were observed at the genotoxic and proteomic level. Differentially expressed proteins (DEPs) were only identified in the B[a]P single exposure and the B[a]P mixture exposure groups containing 1 mg/L of C60, the majority of which were downregulated (~52%). No DEPs were identified at any of the concentrations of nC60 (p < 0.05, 1% FDR). Using DEPs identified at a threshold of (p < 0.05; B[a]P and B[a]P mixture with nC60), gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis indicated that these proteins were enriched with a broad spectrum of biological processes and pathways, including those broadly associated with protein processing, cellular processes and environmental information processing. Among those significantly enriched pathways, the ribosome was consistently the top enriched term irrespective of treatment or concentration and plays an important role as the site of biological protein synthesis and translation. Our results demonstrate the complex multi-modal response to environmental stressors in M. galloprovincialis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Nanomaterials (Basel) Año: 2019 Tipo del documento: Article Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Nanomaterials (Basel) Año: 2019 Tipo del documento: Article Pais de publicación: Suiza