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Physical training, UCP1 expression, mitochondrial density, and coupling in adipose tissue from women with obesity.
Brandao, Camila Fernanda Cunha; de Carvalho, Flavia Giolo; Souza, Anderson de Oliveira; Junqueira-Franco, Márcia Varella Morandi; Batitucci, Gabriela; Couto-Lima, Carlos Antonio; Fett, Carlos Alexandre; Papoti, Marcelo; Freitas, Ellen Cristini de; Alberici, Luciane Carla; Marchini, Julio Sergio.
Afiliación
  • Brandao CFC; Nutrology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
  • de Carvalho FG; School of Physical Education and Sports of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
  • Souza AO; School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
  • Junqueira-Franco MVM; Nutrology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
  • Batitucci G; School of Physical Education and Sports of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
  • Couto-Lima CA; Department of Food and Nutrition, School of Pharmaceutical Sciences of Araraquara, State University of Sao Paulo, Araraquara, Brazil.
  • Fett CA; School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
  • Papoti M; Physical Education Faculty, Federal University of Mato Grosso, Cuiabá, Brazil.
  • Freitas EC; School of Physical Education and Sports of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
  • Alberici LC; School of Physical Education and Sports of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
  • Marchini JS; Department of Food and Nutrition, School of Pharmaceutical Sciences of Araraquara, State University of Sao Paulo, Araraquara, Brazil.
Scand J Med Sci Sports ; 29(11): 1699-1706, 2019 Nov.
Article en En | MEDLINE | ID: mdl-31282585
BACKGROUND: Exercise training may improve energy expenditure, thermogenesis, and oxidative capacities. Therefore, we hypothesized that physical training enhances white adipose tissue mitochondrial oxidative capacity from obese women. OBJECTIVE: To evaluate mitochondrial respiratory capacity, mitochondrial content, and UCP1 gene expression in white adipose tissue from women with obesity before and after the physical training program. METHODS: Women (n = 14, BMI 33 ± 3 kg/m2 , 35 ± 6 years, mean ± SD) were submitted to strength and aerobic exercises (75%-90% maximum heart rate and multiple repetitions), 3 times/week during 8 weeks. All evaluated subjects were paired, before and after training for resting metabolic rate (RMR), substrate oxidation (lipid and carbohydrate) by indirect calorimeter, deuterium oxide body composition, and aerobic maximum velocity (Vmax ) test. At the beginning and at the ending of the protocol, abdominal subcutaneous adipose tissue was collected to measure the mitochondrial respiration by high-resolution respirometry, mitochondrial content by citrate synthase (CS) activity, and UCP1 gene expression by RT-qPCR. RESULTS: Combined physical training increased RMR, lipid oxidation, and Vmax but did not change body weight/composition. In WAT, exercise increased CS activity, decreased mitochondrial uncoupled respiration and mRNA of UCP1. RMR was positively correlated with fat-free mass. CONCLUSION: Physical training promotes an increase in mitochondrial content without changing tissue respiratory capacity, a reduction in mitochondrial uncoupling degree and UCP1 mRNA expression in WAT. Finally, it improved the resting metabolic rate, lipid oxidation and physical performance, independent of the body changing free, or fat mass in obese women.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ejercicio Físico / Tejido Adiposo Blanco / Proteína Desacopladora 1 / Mitocondrias / Obesidad Límite: Adult / Female / Humans Idioma: En Revista: Scand J Med Sci Sports Asunto de la revista: MEDICINA ESPORTIVA Año: 2019 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Dinamarca
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ejercicio Físico / Tejido Adiposo Blanco / Proteína Desacopladora 1 / Mitocondrias / Obesidad Límite: Adult / Female / Humans Idioma: En Revista: Scand J Med Sci Sports Asunto de la revista: MEDICINA ESPORTIVA Año: 2019 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Dinamarca