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Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) Delays Egg Hatching and Larval Development of Progeny.
Chaves, Barbara Aparecida; Junior, Ademir Bentes Vieira; Silveira, Karine Renata Dias; Paz, Andreia da Costa; Vaz, Evelyn Beatriz da Costa; Araujo, Raphaela Guedes Pereira; Rodrigues, Nilton Barnabe; Campolina, Thais Bonifacio; Orfano, Alessandra da Silva; Nacif-Pimenta, Rafael; Villegas, Luis Eduardo Martinez; Melo, Fabrício Freire de; Silva, Breno de Mello; Monteiro, Wuelton Marcelo; Guerra, Maria das Graças Vale Barbosa; Lacerda, Marcus Vinicius Guimarães de; Norris, Douglas Eric; Secundino, Nagila Francinete Costa; Pimenta, Paulo Filemon Paolucci.
Afiliación
  • Chaves BA; Tropical Medicine Foundation Dr. Heitor Vieira Dourado, Manaus, Amazonas, Brazil.
  • Junior ABV; Amazonas State University, Minas Gerais, Belo Horizonte, Brazil.
  • Silveira KRD; Tropical Medicine Foundation Dr. Heitor Vieira Dourado, Manaus, Amazonas, Brazil.
  • Paz ADC; Laboratory of Medical Entomology, Rene Rachou Research Institute - FIOCRUZ, Minas Gerais, Belo Horizonte, Brazil.
  • Vaz EBDC; Tropical Medicine Foundation Dr. Heitor Vieira Dourado, Manaus, Amazonas, Brazil.
  • Araujo RGP; Tropical Medicine Foundation Dr. Heitor Vieira Dourado, Manaus, Amazonas, Brazil.
  • Rodrigues NB; Laboratory of Medical Entomology, Rene Rachou Research Institute - FIOCRUZ, Minas Gerais, Belo Horizonte, Brazil.
  • Campolina TB; Laboratory of Medical Entomology, Rene Rachou Research Institute - FIOCRUZ, Minas Gerais, Belo Horizonte, Brazil.
  • Orfano ADS; Laboratory of Medical Entomology, Rene Rachou Research Institute - FIOCRUZ, Minas Gerais, Belo Horizonte, Brazil.
  • Nacif-Pimenta R; Laboratory of Medical Entomology, Rene Rachou Research Institute - FIOCRUZ, Minas Gerais, Belo Horizonte, Brazil.
  • Villegas LEM; Laboratory of Medical Entomology, Rene Rachou Research Institute - FIOCRUZ, Minas Gerais, Belo Horizonte, Brazil.
  • Melo FF; Laboratory of Medical Entomology, Rene Rachou Research Institute - FIOCRUZ, Minas Gerais, Belo Horizonte, Brazil.
  • Silva BM; Multidisciplinary Health Institute, Federal University of Bahia, Bahia, Brazil.
  • Monteiro WM; Department of Biological Sciences, Federal University of Ouro Preto, Minas Gerais, Ouro Preto, Brazil.
  • Guerra MDGVB; Tropical Medicine Foundation Dr. Heitor Vieira Dourado, Manaus, Amazonas, Brazil.
  • Lacerda MVG; Amazonas State University, Minas Gerais, Belo Horizonte, Brazil.
  • Norris DE; Tropical Medicine Foundation Dr. Heitor Vieira Dourado, Manaus, Amazonas, Brazil.
  • Secundino NFC; Amazonas State University, Minas Gerais, Belo Horizonte, Brazil.
  • Pimenta PFP; Tropical Medicine Foundation Dr. Heitor Vieira Dourado, Manaus, Amazonas, Brazil.
J Med Entomol ; 56(6): 1739-1744, 2019 10 28.
Article en En | MEDLINE | ID: mdl-31278737
Zika virus (ZIKV) has emerged as a globally important arbovirus and has been reported from all states of Brazil. The virus is primarily transmitted to humans through the bite of an infective Aedes aegypti (Linnaeus, 1762) or Aedes albopictus (Skuse, 1895). However, it is important to know if ZIKV transmission also occurs from Ae. aegypti through infected eggs to her offspring. Therefore, a ZIKV and dengue virus (DENV) free colony was established from eggs collected in Manaus and maintained until the third-fourth generation in order to conduct ZIKV vertical transmission (VT) experiments which used an infectious bloodmeal as the route of virus exposure. The eggs from ZIKV-infected females were allowed to hatch. The resulting F1 progeny (larvae, pupae, and adults) were quantitative polymerase chain reaction (qPCR) assayed for ZIKV. The viability of ZIKV vertically transmitted to F1 progeny was evaluated by cultivation in C6/36 cells. The effects of ZIKV on immature development of Ae. aegypti was assessed and compared with noninfected mosquitoes. AmazonianAe. aegypti were highly susceptible to ZIKV infection (96.7%), and viable virus passed to their progeny via VT. Moreover, eggs from the ZIKV-infected mosquitoes had a significantly lower hatch rate and the slowest hatching. In addition, the larval development period was slower when compared to noninfected, control mosquitoes. This is the first study to illustrate VT initiated by oral infection of the parental population by using mosquitoes, which originated from the field and a ZIKV strain that is naturally circulating in-country. Additionally, this study suggests that ZIKV present in the Ae. aegypti can modify the mosquito life cycle. The data reported here suggest that VT of ZIKV to progeny from naturally infected females may have a critical epidemiological role in the dissemination and maintenance of the virus circulating in the vector.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aedes / Virus Zika / Mosquitos Vectores Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: J Med Entomol Año: 2019 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aedes / Virus Zika / Mosquitos Vectores Límite: Animals País/Región como asunto: America do sul / Brasil Idioma: En Revista: J Med Entomol Año: 2019 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido