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Sulfated polysaccharides interact with fibroblast growth factors and protect from denaturation.
Sun, Changye; Liu, Mengxin; Sun, Panwen; Yang, Mingming; Yates, Edwin A; Guo, Zhikun; Fernig, David G.
Afiliación
  • Sun C; Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, China.
  • Liu M; Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, China.
  • Sun P; Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, China.
  • Yang M; Department of Cardiology, School of Medicine, Affiliated Zhongda Hospital, Southeast University, Nanjing, China.
  • Yates EA; Department of Biochemistry, Institute of Integrative Biology, University of Liverpool, UK.
  • Guo Z; Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, China.
  • Fernig DG; Department of Biochemistry, Institute of Integrative Biology, University of Liverpool, UK.
FEBS Open Bio ; 9(8): 1477-1487, 2019 08.
Article en En | MEDLINE | ID: mdl-31271519
Fibroblast growth factors (FGFs) regulate embryonic development and homeostasis, including tissue and organ repair and specific aspects of metabolism. The basic FGF and acidic FGF, now known as FGF2 and FGF1, are widely used protein drugs for tissue repair. However, they are susceptible to denaturation at ambient temperatures and during long-time storage, which will reduce their biological activity. The interaction of FGFs with the sulfated domains of heparan sulfate and heparin is essential for their cellular signaling and stability. Therefore, we analyzed the interactions of FGF1 and FGF2 with four sulfated polysaccharides: heparin, dextran sulfate (DXS), λ-carrageenan, and chondroitin sulfate. The results of thermal stability and cell proliferation assays demonstrate that heparin, DXS, and λ-carrageenan bound to both FGFs and protected them from denaturation. Our results suggest heparin, DXS, and λ-carrageenan are potential formulation materials that bind and stabilize FGFs, and which may also potentiate their activity and control their delivery.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Desnaturalización Proteica / Factor 1 de Crecimiento de Fibroblastos / Factor 2 de Crecimiento de Fibroblastos Límite: Humans Idioma: En Revista: FEBS Open Bio Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Desnaturalización Proteica / Factor 1 de Crecimiento de Fibroblastos / Factor 2 de Crecimiento de Fibroblastos Límite: Humans Idioma: En Revista: FEBS Open Bio Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido