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Commensal and pathogenic biofilms differently modulate peri-implant oral mucosa in an organotypic model.
Ingendoh-Tsakmakidis, Alexandra; Mikolai, Carina; Winkel, Andreas; Szafranski, Szymon P; Falk, Christine S; Rossi, Angela; Walles, Heike; Stiesch, Meike.
Afiliación
  • Ingendoh-Tsakmakidis A; Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover, Germany.
  • Mikolai C; Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover, Germany.
  • Winkel A; Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover, Germany.
  • Szafranski SP; Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover, Germany.
  • Falk CS; Institute of Transplant Immunology, Hannover Medical School, Hannover, Germany.
  • Rossi A; Translational Center for Regenerative Therapies, Fraunhofer Institute of Silicate Research ISC, Würzburg, Germany.
  • Walles H; Translational Center for Regenerative Therapies, Fraunhofer Institute of Silicate Research ISC, Würzburg, Germany.
  • Stiesch M; Chair of Tissue Engineering and Regenerative Medicine, University Hospital of Würzburg, Würzburg, Germany.
Cell Microbiol ; 21(10): e13078, 2019 Oct.
Article en En | MEDLINE | ID: mdl-31270923
The impact of oral commensal and pathogenic bacteria on peri-implant mucosa is not well understood, despite the high prevalence of peri-implant infections. Hence, we investigated responses of the peri-implant mucosa to Streptococcus oralis or Aggregatibacter actinomycetemcomitans biofilms using a novel in vitro peri-implant mucosa-biofilm model. Our 3D model combined three components, organotypic oral mucosa, implant material, and oral biofilm, with structural assembly close to native situation. S. oralis induced a protective stress response in the peri-implant mucosa through upregulation of heat shock protein (HSP70) genes. Attenuated inflammatory response was indicated by reduced cytokine levels of interleukin-6 (IL-6), interleukin-8 (CXCL8), and monocyte chemoattractant protein-1 (CCL2). The inflammatory balance was preserved through increased levels of tumor necrosis factor-alpha (TNF-α). A. actinomycetemcomitans induced downregulation of genes important for cell survival and host inflammatory response. The reduced cytokine levels of chemokine ligand 1 (CXCL1), CXCL8, and CCL2 also indicated a diminished inflammatory response. The induced immune balance by S. oralis may support oral health, whereas the reduced inflammatory response to A. actinomycetemcomitans may provide colonisation advantage and facilitate later tissue invasion. The comprehensive characterisation of peri-implant mucosa-biofilm interactions using our 3D model can provide new knowledge to improve strategies for prevention and therapy of peri-implant disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aggregatibacter actinomycetemcomitans / Modelos Inmunológicos / Biopelículas / Streptococcus oralis / Periimplantitis / Mucosa Bucal Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Cell Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: India

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aggregatibacter actinomycetemcomitans / Modelos Inmunológicos / Biopelículas / Streptococcus oralis / Periimplantitis / Mucosa Bucal Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Cell Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: India