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PulseNet: Entering the Age of Next-Generation Sequencing.
Ribot, Efrain M; Freeman, Molly; Hise, Kelley B; Gerner-Smidt, Peter.
Afiliación
  • Ribot EM; Centers for Disease Control and Prevention, Atlanta, Georgia.
  • Freeman M; Centers for Disease Control and Prevention, Atlanta, Georgia.
  • Hise KB; Centers for Disease Control and Prevention, Atlanta, Georgia.
  • Gerner-Smidt P; Centers for Disease Control and Prevention, Atlanta, Georgia.
Foodborne Pathog Dis ; 16(7): 451-456, 2019 07.
Article en En | MEDLINE | ID: mdl-31241352
Since 1996, PulseNet has served as the national laboratory-based surveillance system for the rapid detection of outbreaks caused by foodborne bacterial pathogens in the United States. For the past two decades, pulsed-field gel electrophoresis was the gold standard subtyping method for the pathogens tracked by PulseNet. A new gold standard is now being implemented with the introduction of cost-effective whole genome sequencing (WGS) for analysis of all the organisms tracked by PulseNet. This transformation is a major undertaking that touches every functional aspect of PulseNet, including laboratory workflows, data storage, analysis management and data interpretation, and language used to communicate information (sequence profile nomenclature system). The benefits of implementing WGS go beyond improved discrimination and precision of the data; it provides an opportunity to determine strain characteristics typically obtained through resource-intensive traditional methodologies, for example, species identification, serotyping, virulence, and antimicrobial resistance profiling, all of which can be consolidated into a single WGS workflow. Such a strategy represents a major shift in the workflows currently practiced in most public health laboratories, but one that brings opportunities for streamlining surveillance activities for the network as a whole. In this study, we provide a brief summary of PulseNet's evolution the past decade along with a general description of the challenges and opportunities that lie ahead.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salud Pública / Brotes de Enfermedades / Secuenciación de Nucleótidos de Alto Rendimiento / Vigilancia en Salud Pública / Enfermedades Transmitidas por los Alimentos Tipo de estudio: Prognostic_studies Aspecto: Determinantes_sociais_saude Límite: Humans País/Región como asunto: America do norte Idioma: En Revista: Foodborne Pathog Dis Asunto de la revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA / PARASITOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Georgia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salud Pública / Brotes de Enfermedades / Secuenciación de Nucleótidos de Alto Rendimiento / Vigilancia en Salud Pública / Enfermedades Transmitidas por los Alimentos Tipo de estudio: Prognostic_studies Aspecto: Determinantes_sociais_saude Límite: Humans País/Región como asunto: America do norte Idioma: En Revista: Foodborne Pathog Dis Asunto de la revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA / PARASITOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Georgia Pais de publicación: Estados Unidos