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LncRNA NEAT1-MicroRNA-140 axis exacerbates nonalcoholic fatty liver through interrupting AMPK/SREBP-1 signaling.
Sun, Yunfu; Song, Yu; Liu, Changsheng; Geng, Jianli.
Afiliación
  • Sun Y; Department of Hepatobiliary Surgery, Weihai Municipal Hospital Affiliated to Dalian Medical University, Weihai, China. Electronic address: yunli34729@163.com.
  • Song Y; Department of Hepatobiliary Surgery, Weihai Municipal Hospital Affiliated to Dalian Medical University, Weihai, China.
  • Liu C; The Second Department of General Surgery, Southern Ward of Weihai Municipal Hospital Affiliated to Dalian Medical University, Weihai, China.
  • Geng J; Department of Hepatobiliary Surgery, Weihai Municipal Hospital Affiliated to Dalian Medical University, Weihai, China.
Biochem Biophys Res Commun ; 516(2): 584-590, 2019 08 20.
Article en En | MEDLINE | ID: mdl-31239155
BACKGROUND: Nonalcoholic fatty liver disease (NAFLD) is a severe liver disease, which influences the health of people worldwide. However, the mechanism modulating the pathogenesis of NAFLD remains elusive. It was reported that nuclear enriched abundant transcript 1 (NEAT1) and microRNA-140 (miR-140) could regulate lipogenesis, but whether they could influence NAFLD are still unknown. METHODS: HepG2 cells were treated by free fatty acids (FFA) to establish the model of NAFLD in vitro, and C57 mice were treated by high-fat diet to establish the model of NAFLD in vivo. Cell transfection was applied to regulate the expression of NEAT1 and miR-140. Western blotting and qRT-PCR were applied for measuring expression of protein and mRNA, respectively. HE staining and Oil Red O staining were used for observing liver tissues. RESULTS: NEAT1 and miR-140 are upregulated in hepacytes under the NAFLD conditions. NEAT1 directly binds to miR-140 and acts synergistically with miR-140 to exacerbate the progression of NAFLD. Reciprocally, silence of miR-140 or NEAT1 alleviates the severity of NAFLD. The mechanistical study shows that the axis of NEAT1-miR-140 inactivates AMPK/SREBP-1 signaling during the NAFLD. . CONCLUSION: The NEAT1-miR-140 axis play a crucial role in modulation of NAFLD via inactivation of AMPK/SREBP1 signaling. This study may provide a novel insight for the treatment of NAFLD.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción de Señal / MicroARNs / Proteína 1 de Unión a los Elementos Reguladores de Esteroles / Proteínas Quinasas Activadas por AMP / ARN Largo no Codificante / Enfermedad del Hígado Graso no Alcohólico Tipo de estudio: Prognostic_studies Límite: Animals / Humans / Male Idioma: En Revista: Biochem Biophys Res Commun Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción de Señal / MicroARNs / Proteína 1 de Unión a los Elementos Reguladores de Esteroles / Proteínas Quinasas Activadas por AMP / ARN Largo no Codificante / Enfermedad del Hígado Graso no Alcohólico Tipo de estudio: Prognostic_studies Límite: Animals / Humans / Male Idioma: En Revista: Biochem Biophys Res Commun Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos