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Comparative Proteomics of Methicillin-Resistant Staphylococcus aureus Subjected to Synergistic Effects of the Lantibiotic Nisin and Oxacillin.
Alves, Fernanda Cristina Bergamo; Albano, Mariana; Andrade, Bruna Fernanda Murbach Teles; Chechi, Jéssica Luana; Pereira, Ana Flávia Marques; Furlanetto, Alessandra; Rall, Vera Lúcia Mores; Fernandes, Ana Angélica Henrique; Dos Santos, Lucilene Delazari; Barbosa, Lidiane Nunes; Fernandes Junior, Ary.
Afiliación
  • Alves FCB; Department of Microbiology and Immunology, Institute of Biosciences of Botucatu (IBB), São Paulo State University (UNESP), Botucatu, Brazil.
  • Albano M; Department of Microbiology and Immunology, Institute of Biosciences of Botucatu (IBB), São Paulo State University (UNESP), Botucatu, Brazil.
  • Andrade BFMT; Department of Microbiology and Immunology, Institute of Biosciences of Botucatu (IBB), São Paulo State University (UNESP), Botucatu, Brazil.
  • Chechi JL; Department of Microbiology and Immunology, Institute of Biosciences of Botucatu (IBB), São Paulo State University (UNESP), Botucatu, Brazil.
  • Pereira AFM; Department of Microbiology and Immunology, Institute of Biosciences of Botucatu (IBB), São Paulo State University (UNESP), Botucatu, Brazil.
  • Furlanetto A; Department of Microbiology and Immunology, Institute of Biosciences of Botucatu (IBB), São Paulo State University (UNESP), Botucatu, Brazil.
  • Rall VLM; Department of Microbiology and Immunology, Institute of Biosciences of Botucatu (IBB), São Paulo State University (UNESP), Botucatu, Brazil.
  • Fernandes AAH; Department of Chemistry and Biochemistry, Institute of Biosciences of Botucatu (IBB), São Paulo State University (UNESP), Botucatu, Brazil.
  • Dos Santos LD; Graduate Program in Tropical Diseases, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
  • Barbosa LN; Center for the Study of Venom and Venomous Animals (CEVAP), São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
  • Fernandes Junior A; Graduate Program in Animal Sciences with Emphasis on Bioactive Products, Universidade Paranaense (UNIPAR), Umuarama, Brazil.
Microb Drug Resist ; 26(3): 179-189, 2020 Mar.
Article en En | MEDLINE | ID: mdl-31237481
We investigated the responses and mechanisms of action of methicillin-resistant Staphylococcus aureus (MRSA) metabolism when exposed under sublethal concentrations of the synergistic antibacterial combination of nisin + oxacillin (» of maximum sublethal concentration) and sublethal concentrations of oxacillin only and nisin only. A total of 135 proteins were identified, showing an alteration in the expression of 85 proteins when treatment was compared with untreated bacteria (control). When the bacteria were treated using the combination, there was an increase in the expression of proteins related to resistance (e.g., beta-lactamase) and also in the ones involved in protein synthesis, and there was a decrease in the expression of proteins related to stress and alterations in proteins related to bacterial energy metabolism. Bacterial oxidative stress showed that the combination was able to induce oxidative stress (p < 0.05) and increase enzyme activities and lipid hydroperoxide levels compared with individual treatments. The analysis of cell ultrastructure showed damage in MRSA, especially on the bacterial wall and the plasma membrane, with cell lysis and death. Thus, the changes caused by these treatments affected different proteins related to the bacterial biological processes and signaling pathways such as cell division, structure, stress, regulation, bacterial resistance, protein synthesis, gene expression, energetic metabolism, and virulence. It was observed that synergism among antimicrobials has high potential in therapeutic use and may reduce the required amounts of antibacterial substances in addition to being effective on different targets in bacterial cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxacilina / Proteínas Bacterianas / Regulación Bacteriana de la Expresión Génica / Staphylococcus aureus Resistente a Meticilina / Antibacterianos / Nisina Idioma: En Revista: Microb Drug Resist Asunto de la revista: MICROBIOLOGIA / TERAPIA POR MEDICAMENTOS Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxacilina / Proteínas Bacterianas / Regulación Bacteriana de la Expresión Génica / Staphylococcus aureus Resistente a Meticilina / Antibacterianos / Nisina Idioma: En Revista: Microb Drug Resist Asunto de la revista: MICROBIOLOGIA / TERAPIA POR MEDICAMENTOS Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos